AbstractA copper(II)-based two-dimensional metal-organic framework with nanosheet structure (CuBDC NS) that possesses peroxidase (POx) mimicking activity was prepared. In the presence of hydrogen peroxide, the system catalyses the oxidation of terephthalic acid to a blue-fluorescent product (excitation = 315 nm; emission = 425 nm). Pyrophosphate has a very strong affinity for Cu2+ ion and blocks the POx-mimicking activity of the CuBDC NS. If, however, inorganic pyrophosphatase is present, the POx mimicking activity is gradually restored because pyrophosphate is hydrolyzed. The findings were used to design a method for the determination of the activity of inorganic pyrophosphatase by fluorometry. Fluorescence increases linearly in the 1–50 mU·mL−1 inorganic pyrophosphatase activity range. The limit of detection is 0.6 mU·mL−1 (S/N = 3). Graphical abstractA copper(II)-based two-dimensional metal-organic framework (CuBDC NS) is described that possesses POx-mimicking activity. Inorganic pyrophosphate (PPi) was hydrolyzed to phosphate in the presence of inorganic pyrophosphatase (PPase). Hence, it cannot coordinate with Cu2+ in CuBDC NS, its structure was well-conserved to catalyses the oxidation of terephthalic acid (H2BDC) to produce a blue fluorescent product (oxBDC) in the presence of hydrogen peroxide (H2O2).

中文翻译：

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利用二维金属有机骨架的过氧化物酶模拟特性荧光测定无机焦磷酸酶及其抑制剂的活性

摘要制备了具有过氧化物酶（POx）模拟活性的具有纳米片结构的铜（II）基二维金属有机骨架（CuBDC NS）。在过氧化氢存在下，该系统催化对苯二甲酸氧化成蓝色荧光产物（激发 = 315 nm；发射 = 425 nm）。焦磷酸盐对 Cu2+ 离子具有非常强的亲和力并阻止 CuBDC NS 的 POx 模拟活性。然而，如果存在无机焦磷酸酶，则由于焦磷酸盐被水解，POx 模拟活性逐渐恢复。研究结果用于设计一种通过荧光法测定无机焦磷酸酶活性的方法。荧光在 1–50 mU·mL-1 无机焦磷酸酶活性范围内线性增加。检测限为 0.6 mU·mL−1 (S/N = 3)。图形摘要描述了具有 POx 模拟活性的基于铜 (II) 的二维金属有机框架 (CuBDC NS)。在无机焦磷酸酶 (PPase) 的存在下，无机焦磷酸 (PPi) 被水解成磷酸盐。因此，它不能与 CuBDC NS 中的 Cu2+ 配位，其结构保守，可在过氧化氢 (H2O2) 存在下催化对苯二甲酸 (H2BDC) 氧化产生蓝色荧光产物 (oxBDC)。