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A Maleimide‐functionalized Tetraphenylethene for Measuring and Imaging Unfolded Proteins in Cells
Chemistry - An Asian Journal ( IF 3.5 ) Pub Date : 2019-02-27 , DOI: 10.1002/asia.201900150 Shouxiang Zhang 1 , Mengjie Liu 1 , Lewis Yi Fong Tan 1 , Quentin Hong 2 , Ze Liang Pow 2 , Tze Cin Owyong 1, 2 , Siyang Ding 1 , Wallace W. H. Wong 2 , Yuning Hong 1
Chemistry - An Asian Journal ( IF 3.5 ) Pub Date : 2019-02-27 , DOI: 10.1002/asia.201900150 Shouxiang Zhang 1 , Mengjie Liu 1 , Lewis Yi Fong Tan 1 , Quentin Hong 2 , Ze Liang Pow 2 , Tze Cin Owyong 1, 2 , Siyang Ding 1 , Wallace W. H. Wong 2 , Yuning Hong 1
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Collapse of the protein homeostasis (proteostasis) can lead to accumulation and aggregation of unfolded proteins, which has been found to associate with a number of disease conditions including neurodegenerative diseases, diabetes and inflammation. Here we report a maleimide‐functionalized tetraphenylethene (TPE)‐derivatized fluorescent dye, TPE‐NMI, which shows fluorescence turn‐on property upon reacting with unfolded proteins in vitro and in live cells under proteostatic stress conditions. The level of unfolded proteins can be measured by flow cytometry and visualized with confocal microscopy.
中文翻译：
马来酰亚胺官能化的四苯乙烯，用于测量和成像细胞中未折叠的蛋白质
蛋白质稳态（蛋白稳态）的崩溃会导致未折叠蛋白质的积累和聚集，这已发现与许多疾病相关，包括神经退行性疾病，糖尿病和炎症。在这里我们报道了一种由马来酰亚胺官能化的四苯乙烯（TPE）衍生的荧光染料TPE-NMI，它在蛋白水解应激条件下与体外和活细胞中未折叠的蛋白反应时显示出荧光开启特性。未折叠蛋白的水平可以通过流式细胞仪测量，并通过共聚焦显微镜观察。
更新日期：2019-02-27
中文翻译：
马来酰亚胺官能化的四苯乙烯，用于测量和成像细胞中未折叠的蛋白质
蛋白质稳态（蛋白稳态）的崩溃会导致未折叠蛋白质的积累和聚集，这已发现与许多疾病相关，包括神经退行性疾病，糖尿病和炎症。在这里我们报道了一种由马来酰亚胺官能化的四苯乙烯（TPE）衍生的荧光染料TPE-NMI，它在蛋白水解应激条件下与体外和活细胞中未折叠的蛋白反应时显示出荧光开启特性。未折叠蛋白的水平可以通过流式细胞仪测量，并通过共聚焦显微镜观察。
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