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A three-dimensional ratiometric sensing strategy on unimolecular fluorescence-thermally activated delayed fluorescence dual emission.
Nature Communications ( IF 14.7 ) Pub Date : 2019-02-13 , DOI: 10.1038/s41467-019-08684-2
Xuping Li 1 , Gleb Baryshnikov 2, 3 , Chao Deng 4 , Xiaoyan Bao 1 , Bin Wu 1 , Yunyun Zhou 1 , Hans Ågren 2, 5 , Liangliang Zhu 1
Nature Communications ( IF 14.7 ) Pub Date : 2019-02-13 , DOI: 10.1038/s41467-019-08684-2
Xuping Li 1 , Gleb Baryshnikov 2, 3 , Chao Deng 4 , Xiaoyan Bao 1 , Bin Wu 1 , Yunyun Zhou 1 , Hans Ågren 2, 5 , Liangliang Zhu 1
Affiliation
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Visualized sensing through fluorescence signals is a powerful method for chemical and physical detection. However, the utilization of fluorescent molecular probes still suffers from lack of precise signal self-calibration in practical use. Here we show that fluorescence and thermally activated delayed fluorescence can be simultaneously produced at the single-molecular level. The thermally activated delayed fluorescence serves as a sensing signal with its wavelength and lifetime both altered correlating to polarity, whereas the fluorescence always remains unchanged as an internal reference. Upon the establishment of a three-dimensional working curve upon the ratiometric wavelength and photoluminescence lifetime vs. polarity, disturbance factors during a relevant sensing process can be largely minimized by such a multiple self-calibration. This strategy was further applied into a precise detection of the microenvironmental polarity variation in complex phospholipid systems, towards providing new insights for convenient and accurate diagnosis of membrane lesions.
中文翻译：
单分子荧光-热激活延迟荧光双重发射的三维比例感测策略。
通过荧光信号进行可视化传感是化学和物理检测的强大方法。然而，在实际使用中，荧光分子探针的使用仍然缺乏精确的信号自校准。在这里，我们显示了可以在单分子水平上同时产生荧光和热激活的延迟荧光。热激活的延迟荧光用作感测信号，其波长和寿命都与极性相关地改变，而荧光始终作为内部参考保持不变。在基于比例波长和光致发光寿命与极性的关系上建立三维工作曲线后，通过这种多重自校准，可以在很大程度上减小相关感测过程中的干扰因素。
更新日期：2019-02-14
中文翻译：
单分子荧光-热激活延迟荧光双重发射的三维比例感测策略。
通过荧光信号进行可视化传感是化学和物理检测的强大方法。然而，在实际使用中，荧光分子探针的使用仍然缺乏精确的信号自校准。在这里，我们显示了可以在单分子水平上同时产生荧光和热激活的延迟荧光。热激活的延迟荧光用作感测信号，其波长和寿命都与极性相关地改变，而荧光始终作为内部参考保持不变。在基于比例波长和光致发光寿命与极性的关系上建立三维工作曲线后，通过这种多重自校准，可以在很大程度上减小相关感测过程中的干扰因素。
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