Herein we investigated the multi-phase distribution and estrogenic effects of endocrine disrupting chemicals (EDCs) in suspended particulate matter (SPM), colloids, and soluble phases from the Shaying River to assess the composition of estrogenic compounds and associated estrogenic risk. The yeast two hybrid (YES) method, cross-flow ultrafiltration (CFUF), and LC-MS/MS were employed. Risk quotient (RQ) values ranged from 0.72 to 3.88, revealing that the Shaying River posed high estrogenic risk to aquatic organisms. The contribution ratios of the target EDCs to the EEQ_YES ranged from 62.7% to 92.5%, indicating that these chemicals were major contributors of estrogenic effects in the Shaying River. Further, 54.0–77.8% of the detected EDCs were distributed in the soluble phase, 15.1–31.7% were bound to colloidal substances, and 3.90–19.4% EDCs were associated with SPM. Significant correlation between total EDC abundance and COD contents was detected, and the concentrations of endogenous estrogens (E1, E2, and E3) were positively correlated with total nitrogen (TN) and total phosphorus (TP). In addition, the in-situ SPM-soluble (Kpoc) and colloid-soluble partition (Kcoc) coefficients were calculated. The log Kpoc values of target compounds varied from 4.10 to 5.19, while log Kcoc values ranged from 4.25 to 5.56. Their Kcoc values were larger than the Kpoc values, indicating that organic colloids were the most important carriers of EDCs in the aquatic environment.

本文中，我们研究了来自沙ying河的悬浮颗粒物（SPM），胶体和可溶性相中内分泌干扰物（EDC）的多相分布和雌激素作用，以评估雌激素化合物的组成和相关的雌激素风险。采用了酵母两种杂交（YES）方法，错流超滤（CFUF）和LC-MS / MS。风险商（RQ）值介于0.72至3.88之间，这表明沙River河对水生生物构成高雌激素风险。目标EDC对EEQ的贡献率_是范围从62.7％到92.5％，表明这些化学物质是沙ying河雌激素作用的主要贡献者。此外，检测到的EDC中有54.0–77.8％分布在可溶相中，与胶体物质结合的占15.1–31.7％，SPM有3.90–19.4％的EDC。检测到总EDC含量与COD含量之间存在显着相关性，内源性雌激素（E1，E2和E3）的浓度与总氮（TN）和总磷（TP）正相关。此外，计算了原位SPM可溶性（Kpoc）和胶体可溶性分配（Kcoc）系数。目标化合物的log Kpoc值在4.10至5.19之间变化，而log Kcoc值在4.25至5.56之间变化。它们的Kcoc值大于Kpoc值，