当前位置：
X-MOL 学术
›
J. Agric. Food Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Prevention of Obesity and Hyperlipidemia by Heptamethoxyflavone in High-fat Diet-induced Rats
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-02-11 00:00:00 , DOI: 10.1021/acs.jafc.8b05632 Konglong Feng 1 , Xiaoai Zhu 1 , Tong Chen 1, 2 , Bo Peng 1, 3 , Muwen Lu 1, 4 , Hui Zheng 5 , Qingrong Huang 6 , Chi-Tang Ho 6 , Yunjiao Chen 1, 4 , Yong Cao 1, 4
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-02-11 00:00:00 , DOI: 10.1021/acs.jafc.8b05632 Konglong Feng 1 , Xiaoai Zhu 1 , Tong Chen 1, 2 , Bo Peng 1, 3 , Muwen Lu 1, 4 , Hui Zheng 5 , Qingrong Huang 6 , Chi-Tang Ho 6 , Yunjiao Chen 1, 4 , Yong Cao 1, 4
Affiliation
|
Polymethoxyflavones (PMFs) have been shown to prevent obesity, ameliorate type 2 diabetes, and regulate lipid metabolism in vitro and in vivo. However, little is known about the contribution of 3,5,6,7,8,3′,4′-heptamethoxyflavone (HMF) to prevent obesity and regulate lipid metabolism in vivo. We aimed to investigate the potential efficacy of HMF on preventing obesity and hyperlipidemia in rats fed a high-fat diet (HFD) and its underlying mechanisms. Male Sprague–Dawley rats were fed a normal diet or an HFD with or without HMF (0.02%, 0.04% and 0.08%, w/w) for 6 weeks. The supplementation of HMF not only significantly decreased body weight gain (HFD, 336.50 ± 18.84 g; LHMF, 309.43 ± 20.74 g; MHMF, 296.83 ± 13.88 g; HHMF, 265.71 ± 19.09 g; respectively, p < 0.05) and adipose tissues weight (p < 0.05), but also markedly lowered serum levels of total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol (p < 0.05) in the sixth week in a dose-dependent manner compared with the HFD group. HMF also significantly alleviated hepatic steatosis in the liver (liver weight g/100 g body weight of HFD, 4.86 ± 0.11%; LHMF, 4.02 ± 0.33%; MHMF, 4.05 ± 0.31%; HHMF, 3.72 ± 0.34%; respectively, p < 0.05). Furthermore, transcriptome analysis and real-time quantitative RT-PCR demonstrated that HMF supplementation markedly downregulated hepatic genes related to adipogenesis transcription and inflammatory responses, and significantly upregulated genes related to fatty acid oxidation and energy expenditure. These results indicated that HMF could effectively prevent obesity and hyperlipidemia by regulation of the expression of lipid metabolism-related and inflammatory response-related genes.
中文翻译：
七甲氧基黄酮对高脂饮食诱导的大鼠肥胖和高脂血症的预防
聚甲氧基黄酮（PMF）已被证明可以预防肥胖，改善2型糖尿病并在体内和体外调节脂质代谢。然而，关于3,5,6,7,8,3'，4'-七甲氧基黄酮（HMF）在体内预防肥胖和调节脂质代谢的贡献知之甚少。我们旨在研究HMF预防高脂饮食（HFD）喂养的大鼠肥胖和高脂血症的潜在功效及其潜在机制。对Sprague–Dawley雄性大鼠喂食正常饮食或HFD，伴或不伴HMF（0.02％，0.04％和0.08％，w / w），为期6周。补充HMF不仅显着降低了体重增加（HFD，336.50±18.84 g; LHMF，309.43±20.74 g; MHMF，296.83±13.88 g; HHMF，265.71±19.09 g;p <0.05）和脂肪组织的重量（p <0.05），但与第6周相比，第6周的血清总胆固醇，三酰甘油和低密度脂蛋白胆固醇水平显着降低（p <0.05）。 HFD组。HMF还可以显着减轻肝脏的肝脂肪变性（肝重量g / 100 g HFD体重，4.86±0.11％; LHMF，4.02±0.33％; MHMF，4.05±0.31％; HHMF，3.72±0.34％; p<0.05）。此外，转录组分析和实时定量RT-PCR表明，补充HMF显着下调了与脂肪形成转录和炎症反应有关的肝基因，并显着上调了与脂肪酸氧化和能量消耗有关的基因。这些结果表明，HMF可以通过调节脂质代谢相关基因和炎症反应相关基因的表达来有效预防肥胖和高脂血症。
更新日期：2019-02-11
中文翻译：
七甲氧基黄酮对高脂饮食诱导的大鼠肥胖和高脂血症的预防
聚甲氧基黄酮（PMF）已被证明可以预防肥胖，改善2型糖尿病并在体内和体外调节脂质代谢。然而，关于3,5,6,7,8,3'，4'-七甲氧基黄酮（HMF）在体内预防肥胖和调节脂质代谢的贡献知之甚少。我们旨在研究HMF预防高脂饮食（HFD）喂养的大鼠肥胖和高脂血症的潜在功效及其潜在机制。对Sprague–Dawley雄性大鼠喂食正常饮食或HFD，伴或不伴HMF（0.02％，0.04％和0.08％，w / w），为期6周。补充HMF不仅显着降低了体重增加（HFD，336.50±18.84 g; LHMF，309.43±20.74 g; MHMF，296.83±13.88 g; HHMF，265.71±19.09 g;p <0.05）和脂肪组织的重量（p <0.05），但与第6周相比，第6周的血清总胆固醇，三酰甘油和低密度脂蛋白胆固醇水平显着降低（p <0.05）。 HFD组。HMF还可以显着减轻肝脏的肝脂肪变性（肝重量g / 100 g HFD体重，4.86±0.11％; LHMF，4.02±0.33％; MHMF，4.05±0.31％; HHMF，3.72±0.34％; p<0.05）。此外，转录组分析和实时定量RT-PCR表明，补充HMF显着下调了与脂肪形成转录和炎症反应有关的肝基因，并显着上调了与脂肪酸氧化和能量消耗有关的基因。这些结果表明，HMF可以通过调节脂质代谢相关基因和炎症反应相关基因的表达来有效预防肥胖和高脂血症。
京公网安备 11010802027423号