The genus of Parasutterella has been defined as a core component of the human and mouse gut microbiota, and has been correlated with various health outcomes. However, like most core microbes in the gastrointestinal tract (GIT), very little is known about the biology of Parasutterella and its role in intestinal ecology. In this study, Parasutterella was isolated from the mouse GIT and characterized in vitro and in vivo. Mouse, rat, and human Parasutterella isolates were all asaccharolytic and producers of succinate. The murine isolate stably colonized the mouse GIT without shifting bacterial composition. Notable changes in microbial-derived metabolites were aromatic amino acid, bilirubin, purine, and bile acid derivatives. The impacted bile acid profile was consistent with altered expression of ileal bile acid transporter genes and hepatic bile acid synthesis genes, supporting the potential role of Parasutterella in bile acid maintenance and cholesterol metabolism. The successful colonization of Parasutterella with a single environmental exposure to conventional adult mice demonstrates that it fills the ecological niche in the GIT and contributes to metabolic functionalities. This experiment provides the first indication of the role of Parasutterella in the GIT, beyond correlation, and provides insight into how it may contribute to host health.

Parasutterella属已被定义为人和小鼠肠道微生物群的核心组成部分，并与各种健康结果相关。然而，与胃肠道 (GIT) 中的大多数核心微生物一样，对Parasutterella的生物学及其在肠道生态学中的作用知之甚少。在这项研究中，Parasutterella从小鼠胃肠道中分离出来，并在体外和体内进行了表征。小鼠、大鼠和人类Parasutterella分离株都是无糖分解和琥珀酸盐的生产者。小鼠分离物稳定地定植于小鼠胃肠道，而不会改变细菌组成。微生物衍生代谢物的显着变化是芳香族氨基酸、胆红素、嘌呤和胆汁酸衍生物。受影响的胆汁酸谱与回肠胆汁酸转运蛋白基因和肝胆汁酸合成基因的表达改变一致，支持Parasutterella在胆汁酸维持和胆固醇代谢中的潜在作用。Parasutterella的成功定植对常规成年小鼠的单一环境暴露表明它填补了 GIT 中的生态位并有助于代谢功能。该实验首次表明Parasutterella在 GIT中的作用，超越相关性，并深入了解它如何促进宿主健康。