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ROS Is a Factor Regulating the Increased Polysaccharide Production by Light Quality in the Edible Cyanobacterium Nostoc flagelliforme
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-02-06 00:00:00 , DOI: 10.1021/acs.jafc.8b06176 Pei-pei Han 1 , Shi-gang Shen 1 , Rong-jun Guo 1 , Dong-xue Zhao 1 , Ya-Hui Lin 1 , Shi-ru Jia 1 , Rong-rong Yan 1 , Yi-kai Wu 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-02-06 00:00:00 , DOI: 10.1021/acs.jafc.8b06176 Pei-pei Han 1 , Shi-gang Shen 1 , Rong-jun Guo 1 , Dong-xue Zhao 1 , Ya-Hui Lin 1 , Shi-ru Jia 1 , Rong-rong Yan 1 , Yi-kai Wu 1
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To explore the regulatory factor of light quality affecting exopolysaccharide (EPS) production, transcriptome analysis of Nostoc flagelliforme cells exposed to red light (R), blue light (B), and mixed light (B/R = 15:7) (BR) with white fluorescent light as control was performed. The differentially expressed genes mainly enriched in carbohydrate metabolism and energy metabolism. Significant enrichment in the oxidation–reduction process and energy metabolism indicated that intracellular redox homeostasis was disrupted. An assay of reactive oxygen species (ROS) and malondialdehyde contents demonstrated light quality induced oxidative stress. To illustrate the relationship between ROS level and EPS accumulation, the effects of the exogenous addition of ROS scavenger N-acetyl cysteine and inducer H2O2 on the oxidation–reduction level and EPS production were compared. The results revealed that light quality regulated EPS biosynthesis via the intracellular ROS level directly other than oxidative stress. Understanding such relationships might provide guidance for efficient EPS production to regulate the intracellular redox level.
中文翻译：
ROS是调节食用蓝藻Nostoc鞭毛状菌中光质量增加的多糖生产的调节因子。
为了探索影响外多糖（EPS）产生的光质量调节因子，对暴露于红光（R），蓝光（B）和混合光（B / R = 15：7）（BR）的鞭毛鞭毛状细胞的转录组分析用白色荧光灯作为对照。差异表达的基因主要富含碳水化合物代谢和能量代谢。氧化还原过程和能量代谢的大量富集表明细胞内氧化还原稳态被破坏。活性氧（ROS）和丙二醛含量的测定表明，光质诱导了氧化应激。为了说明ROS水平与EPS积累之间的关系，外源添加ROS清除剂N-乙酰半胱氨酸和诱导剂H 2的影响比较了O 2的氧化还原水平和EPS生成量。结果表明，光质量通过细胞内ROS水平直接调节除氧化应激外的EPS生物合成。了解这种关系可能为有效的EPS生产提供指导，以调节细胞内的氧化还原水平。
更新日期：2019-02-06
中文翻译：
ROS是调节食用蓝藻Nostoc鞭毛状菌中光质量增加的多糖生产的调节因子。
为了探索影响外多糖（EPS）产生的光质量调节因子，对暴露于红光（R），蓝光（B）和混合光（B / R = 15：7）（BR）的鞭毛鞭毛状细胞的转录组分析用白色荧光灯作为对照。差异表达的基因主要富含碳水化合物代谢和能量代谢。氧化还原过程和能量代谢的大量富集表明细胞内氧化还原稳态被破坏。活性氧（ROS）和丙二醛含量的测定表明，光质诱导了氧化应激。为了说明ROS水平与EPS积累之间的关系，外源添加ROS清除剂N-乙酰半胱氨酸和诱导剂H 2的影响比较了O 2的氧化还原水平和EPS生成量。结果表明，光质量通过细胞内ROS水平直接调节除氧化应激外的EPS生物合成。了解这种关系可能为有效的EPS生产提供指导，以调节细胞内的氧化还原水平。
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