Formation of Protein Corona on Nanoparticles with Digestive Enzymes in Simulated Gastrointestinal Fluids,Journal of Agricultural and Food Chemistry

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Formation of Protein Corona on Nanoparticles with Digestive Enzymes in Simulated Gastrointestinal Fluids
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-02-05 00:00:00 , DOI: 10.1021/acs.jafc.8b05702
Yihui Wang ₁ , Man Li ₁ , Xingfeng Xu ₁ , Wenting Tang ₁ , Liu Xiong ₁ , Qingjie Sun ₁

Affiliation

The protein corona (PC), which defines the biological identity of nanoparticles in the blood, is well-known, but no comprehensive and systematic study has been conducted yet on the formation of PCs in the gastrointestinal environment. Thus, this study aimed to explore the interaction between model polystyrene nanoparticles (PS-NPs) of 50–100 nm and three digestive enzymes, namely, pepsin, α-amylase, and trypsin. Results showed that the thicknesses of the PCs formed by α-amylase and trypsin were 25–100 and 50–100 nm, respectively. The zeta-potential values of PS-NPs after incubation significantly increased. The fluorescence quenching and ultraviolet–visible-absorption spectra suggested that interactions between the nanoparticles and the enzymes occurred. Synchronous fluorescence spectra showed that the PS-NPs could induce microenvironmental changes in digestive enzymes. The thermodynamic parameters suggested that the interaction was mainly driven by hydrogen bonds and van der Waals forces.

中文翻译：

在模拟胃肠液中具有消化酶的纳米粒子上蛋白质电晕的形成。

定义血液中纳米颗粒的生物学特性的蛋白质电晕（PC）是众所周知的，但是尚未对胃肠道环境中PC的形成进行全面而系统的研究。因此，本研究旨在探讨50–100 nm的模型聚苯乙烯纳米颗粒（PS-NP）与三种消化酶，即胃蛋白酶，α-淀粉酶和胰蛋白酶之间的相互作用。结果表明，由α-淀粉酶和胰蛋白酶形成的PC的厚度分别为25-100 nm和50-100 nm。孵育后PS-NP的Zeta电位值显着增加。荧光猝灭和紫外可见吸收光谱表明，纳米颗粒和酶之间发生了相互作用。同步荧光光谱表明，PS-NPs可以诱导消化酶的微环境变化。热力学参数表明相互作用主要是由氢键和范德华力驱动的。

更新日期：2019-02-05

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