Reversibly Assembled Electroconductive Hydrogel via a Host–Guest Interaction for 3D Cell Culture,ACS Applied Materials & Interfaces

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Reversibly Assembled Electroconductive Hydrogel via a Host–Guest Interaction for 3D Cell Culture
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2019-02-04 00:00:00 , DOI: 10.1021/acsami.8b19482
Yong Xu ₁ , Meiying Cui ₁ , Panagiotis A. Patsis ₁ , Markus Günther ₂ , Xuegeng Yang ₃ , Kerstin Eckert ₃ , Yixin Zhang ₁

Affiliation

The study of cells responding to an electroconductive environment is impeded by the lack of a method, which would allow the encapsulation of cells in an extracellular matrix-like 3D electroactive matrix, and more challengingly, permit a simple mechanism to release cells for further characterization. Herein, we report a polysaccharide-based conductive hydrogel system formed via a β-cyclodextrin-adamantane host–guest interaction. Oxidative polymerization of 3,4-ethylenedioxythiophene (EDOT) in the presence of adamantyl-modified sulfated alginate (S-Alg-Ad) results in bio-electroconductive polymer PEDOT:S-Alg-Ad, which can form hydrogel with poly-β-cyclodextrin (Pβ-CD). The PEDOT:S-Alg-Ad/Pβ-CD hydrogels can be tuned on aspects of mechanical and electrical properties, exhibit self-healing feature, and are injectable. Electron microscopy suggested that the difference in stiffness and conductivity is associated with the nacre-like layered nanostructures when different sizes of PEDOT:S-Alg-Ad nanoparticles were used. Myoblast C2C12 cells were encapsulated in the conductive hydrogel and exhibited proliferation rate comparable to that in nonconductive S-Alg-Ad/Pβ-CD hydrogel. The cells could be released from the hydrogels by adding the β-CD monomer. Astonishingly, the conductive hydrogel can dramatically promote myotube-like structure formation, which is not in the non-electroconductive hydrogel. The ability to embed and release cells in an electroconductive environment will open new doors for cell culture and tissue engineering.

中文翻译：

通过宿主-来宾相互作用可逆组装的导电水凝胶，用于3D细胞培养

缺乏对方法的研究阻碍了对导电环境做出反应的研究，该方法可以将细胞封装在类似细胞外基质的3D电活性基质中，而且更具挑战性的是，可以采用简单的机制释放细胞以进行进一步表征。在本文中，我们报道了通过β-环糊精-金刚烷宿主-客体相互作用形成的基于多糖的导电水凝胶系统。在金刚烷基改性的硫酸化藻酸盐（S-Alg-Ad）存在下，3,4-乙撑二氧噻吩（EDOT）的氧化聚合产生生物导电聚合物PEDOT：S-Alg-Ad，该聚合物可与聚-β-形成水凝胶环糊精（Pβ-CD）。PEDOT：S-Alg-Ad /Pβ-CD水凝胶可以在机械和电气特性方面进行调整，具有自我修复功能，并且可以注射。电子显微镜表明，当使用不同尺寸的PEDOT：S-Alg-Ad纳米粒子时，刚度和电导率的差异与珍珠层状纳米结构有关。成肌细胞C2C12细胞被封装在导电水凝胶中，并显示出与非导电S-Alg-Ad /Pβ-CD水凝胶相当的增殖速率。可以通过添加β-CD单体将细胞从水凝胶中释放出来。令人惊讶的是，导电性水凝胶可以显着促进肌管状结构的形成，这是非导电性水凝胶所没有的。在导电环境中嵌入和释放细胞的能力将为细胞培养和组织工程打开新的大门。使用了S-Alg-Ad纳米颗粒。成肌细胞C2C12细胞被封装在导电水凝胶中，并表现出与非导电S-Alg-Ad /Pβ-CD水凝胶相当的增殖速率。可以通过添加β-CD单体将细胞从水凝胶中释放出来。令人惊讶的是，导电性水凝胶可以显着促进肌管状结构的形成，这是非导电性水凝胶所没有的。在导电环境中嵌入和释放细胞的能力将为细胞培养和组织工程打开新的大门。使用了S-Alg-Ad纳米颗粒。成肌细胞C2C12细胞被封装在导电水凝胶中，并表现出与非导电S-Alg-Ad /Pβ-CD水凝胶相当的增殖速率。可以通过添加β-CD单体将细胞从水凝胶中释放出来。令人惊讶的是，导电性水凝胶可以显着促进肌管状结构的形成，这是非导电性水凝胶所没有的。在导电环境中嵌入和释放细胞的能力将为细胞培养和组织工程打开新的大门。导电水凝胶可以显着促进肌管状结构的形成，这是非导电水凝胶所没有的。在导电环境中嵌入和释放细胞的能力将为细胞培养和组织工程打开新的大门。导电水凝胶可以显着促进肌管状结构的形成，这是非导电水凝胶所没有的。在导电环境中嵌入和释放细胞的能力将为细胞培养和组织工程打开新的大门。

更新日期：2019-02-04

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