Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide–Peptide Interaction,Journal of Agricultural and Food Chemistry

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Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide–Peptide Interaction
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2019-02-05 00:00:00 , DOI: 10.1021/acs.jafc.8b07109
Kaining Han ₁ , Danyang Luo ₁ , Yuan Zou ₂ , Shiyuan Dong ₃ , Zhili Wan ₁ , Xiaoquan Yang _{1,

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Affiliation

Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders. This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)–peptide interaction. In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria. ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae. Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca²⁺ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers. This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.

中文翻译：

涉及脂多糖-肽相互作用的大豆7S球蛋白肽对肠道菌群的调节

大豆蛋白对控制代谢综合征具有营养意义，并且证据表明肠道菌群与代谢紊乱的控制有关。这项研究旨在研究大豆7S球蛋白胃蛋白酶释放肽对肠道菌群的调节作用，以及肠道菌群组成的变化与脂多糖（LPS）-肽相互作用之间的可能联系。体外发酵实验表明，大豆7S球蛋白的延伸区（ER）片段选择性抑制促炎性革兰氏阴性细菌。ER肽还促进了短链脂肪酸（SCFAs）的最高产生，这与链霉菌科和乳杆菌科的相对丰度增加有关。²⁺并发展成为富含β-折叠的聚集体结构，从而削弱了LPS单层的稳定性。该发现为改善大豆7S球蛋白对代谢疾病的作用提供了可能的解释。

更新日期：2019-02-05

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