The purpose of this study was to investigate the pharmacokinetics of the polymethoxylated flavonoids kumatakenin, pachypodol, and retusin, which contain two, three, or four methoxy substitutions, using a validated ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) method in rats. The pharmacokinetic results demonstrated that the elimination half-lives for kumatakenin, pachypodol, and retusin were 30 ± 11.6, 39.4 ± 19.5, and 106.9 ± 26 min, respectively, for the low dose group and 54.5 ± 16.5, 33.8 ± 10, and 134.6 ± 34.7 min for the high dose group. The results suggested that the area under the curve values (AUC) for the analytes did not correlate with the number of methoxy groups. Pachypodol had the lowest AUC, which may have been correlated with lipophilicity, for both the low and high dose groups. In conclusion, the polymethoxylated flavonoid pachypodol is more hydrophilic than kumatakenin or retusin, which were correlated with the pharmacokinetic results.

中文翻译：

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HPLC-MS / MS法测定大鼠血浆中聚甲氧基黄酮的结构药代动力学

这项研究的目的是使用经过验证的超高效液相色谱-串联质谱法（UHPLC-MS）研究多甲氧基化黄酮类化合物kumatakenin，pachypodol和retusin的药代动力学，其中含有两个，三个或四个甲氧基取代基。 / MS）方法。药代动力学结果表明，低剂量组和kumatakenin，pachypodol和retusin的消除半衰期分别为30±11.6、39.4±19.5和106.9±26 min，分别为54.5±16.5、33.8±10和134.6。高剂量组±34.7分钟。结果表明，分析物的曲线值下面积（AUC）与甲氧基的数量不相关。对于低剂量组和高剂量组，帕西波多的AUC最低，这可能与亲脂性有关。综上所述，