In this study, a simple, sensitive and reliable gas chromatography-flame ionization detection (GC-FID) method is established for quantitative chemical fingerprinting of essential oils from Pogostemon cablin. Oil samples are prepared by hydrodistillation, with yields ranging from 0.73% to 2.02%. The two main components of the oil, patchouli alcohol and pogostone, were detected simultaneously in 36 samples and were found to have average contents of 43.07% and 7.84%, respectively. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. All calibration curves showed excellent linearity (r² > 0.9992) within the test ranges, and the relative standard deviation (RSD) values for intra- and inter-day precision were less than 1.5%, indicating a high degree of precision. The GC-FID chemical fingerprints of the 36 samples were established using 12 common peaks which account for over 90% of the total peak area. Chemometric techniques, including similarity analysis and hierarchical cluster analysis, were also employed to explore the similarities and outstanding consistencies among different P. cablin oil samples. The results demonstrate that chromatographic fingerprinting and quantitative analysis can be achieved simultaneously when evaluating quality and authenticating samples of P. cablin.

在这项研究中，建立了一种简单，灵敏，可靠的气相色谱-火焰电离检测（GC-FID）方法，用于定量分析来自Pogostemon cablin的香精油的化学指纹图谱。通过加氢蒸馏制备油样品，产率为0.73％至2.02％。在36个样品中同时检测到了油中的两种主要成分广patch香醇和Pogostone，其平均含量分别为43.07％和7.84％。该方法在线性，灵敏度，精度，稳定性和准确性方面得到了验证。所有校准曲线均显示出出色的线性度（r ² > 0.9992），并且日内和日间精度的相对标准偏差（RSD）值均小于1.5％，表明具有很高的精度。使用12个共同峰建立了36个样品的GC-FID化学指纹图谱，这些峰占总峰面积的90％以上。化学计量学技术，包括相似性分析和层次聚类分析，也被用来探索不同的卡宾油样品之间的相似性和突出的一致性。结果表明，在评价卡氏假单胞菌的质量和鉴定样品时，可以同时进行色谱指纹图谱和定量分析。