1,3-二苯基异苯并呋喃的开发作为一种高选择性探针，用于检测和定量测定过氧化氢。,Free Radical Research

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1,3-二苯基异苯并呋喃的开发作为一种高选择性探针，用于检测和定量测定过氧化氢。
Free Radical Research ( IF 3.6 ) Pub Date : 2016 Dec 13 , DOI: 10.1080/10715762.2016.1262541
Krzysztof Żamojć ₁ , Magdalena Zdrowowicz ₁ , Paweł Błażej Rudnicki-Velasquez ₁ , Karol Krzymiński ₁ , Bartłomiej Zaborowski ₁ , Paweł Niedziałkowski ₁ , Dagmara Jacewicz ₁ , Lech Chmurzyński ₁

Affiliation

1,3-二苯基异苯并呋喃（DPBF）已开发为选择性探针，用于检测和定量测定包含不同活性氮和氧种类（RNOS）的样品中的过氧化氢。DPBF是一种荧光探针，在近20年的时间里，它被认为可以以高度特异性的方式与某些活性氧（ROS）反应，例如单线态氧和羟基，烷氧基或烷基过氧自由基。在这些个体的作用下，DPBF已迅速转化为1,2-二苯甲酰苯（DBB）。为了检查DPBF是否可以作为不同RNOS总量以及这些个体过度生产所引起的氧化应激的唯一指标，进行了一系列实验，其中DPBF与过氧亚硝酸盐阴离子，超氧化物反应阴离子，过氧化氢，次氯酸根阴离子，以及通常在生物条件下存在的阴离子，即亚硝酸盐和硝酸盐。在所有情况下，除过氧化氢外，反应产物均为DBB。仅在H 2 O 2的作用下，形成9-羟基蒽-10（9H）-一（恶蒽酮）。该产品已通过使用荧光光谱，NMR光谱，高效液相色谱与质谱，红外光谱，元素分析和循环伏安法（CV）进行鉴定。发现DPBF的荧光强度的降低与过氧化氢的浓度在0.196-3.941mM的范围内呈线性关系。DPBF以非常特定的方式响应过氧化氢，其检测和定量限分别为88和122.8μM。

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The development of 1,3-diphenylisobenzofuran as a highly selective probe for the detection and quantitative determination of hydrogen peroxide.

1,3-Diphenylisobenzofuran (DPBF) has been developed as a selective probe for the detection and quantitative determination of hydrogen peroxide in samples containing different reactive nitrogen and oxygen species (RNOS). DPBF is a fluorescent probe which, for almost 20 years, was believed to react in a highly specific manner toward some reactive oxygen species (ROS) such as singlet oxygen and hydroxy, alkyloxy or alkylperoxy radicals. Under the action of these individuals DPBF has been rapidly transformed to 1,2-dibenzoylbenzene (DBB). In order to check if DPBF can act as a unique indicator of the total amount of different RNOS, as well as oxidative stress caused by an overproduction of these individuals, a series of experiments was carried out, in which DPBF reacted with peroxynitrite anion, superoxide anion, hydrogen peroxide, hypochlorite anion, and anions commonly present under biological conditions, namely nitrite and nitrate. In all cases, except for hydrogen peroxide, the product of the reaction is DBB. Only under the action of H2O2 9-hydroxyanthracen-10(9H)-one (oxanthrone) is formed. This product has been identified with the use of fluorescence spectroscopy, NMR spectroscopy, high performance liquid chromatography coupled with mass spectrometry, infrared spectroscopy, elemental analysis, and cyclic voltammetry (CV). A linear relationship was found between a decrease in the fluorescence intensity of DPBF and the concentration of hydrogen peroxide in the range of concentrations of 0.196-3.941 mM. DPBF responds to hydrogen peroxide in a very specific way with the limits of detection and quantitation of 88 and 122.8 muM, respectively. The kinetics of the reaction between DBBF and H2O2 was also studied.

更新日期：2017-01-31

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