Surfaces that facilitate selective cell adhesion using specific targeting moieties have great implications in diagnostics, tissue engineering, and high throughput screenings. However, designing robust and spatially confined micropatterns for selective cell isolation on portable platform is highly challenging. Here, wettable, silane (Si) micropatterns holding covalently attached transferrin (Tf) for targeting Tf overexpressing cancer cells are reported. These micropatterns are separated by carbon soot based superhydrophobic regions that turn these targeting sites into surface tension confined “microwells.” These microwells facilitate capture of human colorectal carcinoma cells (HCT 116) and human cervical adenocarcinoma cells (HeLa) by confining their attachment to wettable region, thereby making isolation and spotting of the targeted cells more efficient. In addition, owing to its transparent trait, the Tf conjugated wettability based patterned chip offer real time optical monitoring of cell adhesion, cell growth, and cell behavior. The specific cell isolation using such surface has applications in devising cancer recurrence monitoring tests.

中文翻译：

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转铁蛋白官能化的硅烷-碳黑介导的超疏水微图案选择性细胞分离

使用特定的靶向部分促进选择性细胞粘附的表面在诊断，组织工程和高通量筛选中具有重大意义。然而，设计用于在便携式平台上进行选择性细胞分离的鲁棒且空间受限的微图案是极富挑战性的。在此，报道了可湿性的，具有共价连接的转铁蛋白（Tf）的硅烷（Si）微图案用于靶向过表达Tf的癌细胞。这些微图案被基于碳烟尘的超疏水区域隔开，这些区域将这些目标位置转变为受表面张力限制的“微孔”。这些微孔通过限制它们与可润湿区域的附着，从而促进了人类大肠癌细胞（HCT 116）和人类宫颈腺癌细胞（HeLa）的捕获，从而使靶细胞的分离和定位更加有效。此外，由于其透明特性，基于Tf共轭可湿性的图案化芯片可对细胞粘附，细胞生长和细胞行为进行实时光学监控。使用这种表面的特定细胞分离在设计癌症复发监测测试中具有应用。