<br>高效液相色谱-电喷雾串联质谱法同时分析马尾藻中的10种植物激素,Journal of Separation Science

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高效液相色谱-电喷雾串联质谱法同时分析马尾藻中的10种植物激素
Journal of Separation Science ( IF 2.8 ) Pub Date : 2016-05-03 , DOI: 10.1002/jssc.201501239
Yan Li ₁ , Chengxu Zhou ₁ , Xiaojun Yan ₁ , Jinrong Zhang ₁ , Jilin Xu ₂

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植物激素因其重要的生物学功能而受到广泛关注。然而，由于其成分复杂、丰度低且来源多样，其检测仍然是一个挑战。在这项研究中，开发并验证了一种高效液相色谱-电喷雾电离串联质谱的新方法，用于同时测定十种植物激素，包括吲哚-3-乙酸、异戊烯基腺嘌呤、异戊烯基腺苷、反式玉米素核苷、玉米素、马尾藻( S. horneri ) 中的独脚金内酯、脱落酸、水杨酸、赤霉素 A3 和茉莉酸。使用甲醇/水/甲酸（15:4:1，v/v/v）从冻干的S. horneri中提取植物激素，在 Hypersil Gold C ₁₈柱上进行分析，并通过电喷雾电离串联三重四极杆质谱法进行检测。多重反应监测模式。对植物激素提取和分析的实验条件进行了优化，并在重现性、线性、灵敏度、回收率、准确性和稳定性方面进行了验证。首次研究了S. horneri在漂移、固定和半固定生长状态下的茎、叶片和气囊中植物激素的分布。所观察到的S. horneri中植物激素的含量范围从未检出到5066.67 ng/g（鲜重）。大多数植物激素主要以漂移和半固定状态分布在S. horneri的茎中。

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Simultaneous analysis of ten phytohormones in Sargassum horneri by high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry

Phytohormones have attracted wide attention due to their important biological functions. However, their detection is still a challenge because of their complex composition, low abundance and diverse sources. In this study, a novel method of high‐performance liquid chromatography with electrospray ionization tandem mass spectrometry was developed and validated for the simultaneous determination of ten phytohormones including indole‐3‐acetic acid, isopentenyladenine, isopentenyl adenosine, trans‐zeatin riboside, zeatin, strigolactones, abscisic acid, salicylic acid, gibberellin A3, and jasmonic acid in Sargassum horneri (S. horneri). The phytohormones were extracted from freeze‐dried S. horneri with methanol/water/methanoic acid (15:4:1, v/v/v) analyzed on a Hypersil Gold C₁₈ column and detected by electrospray ionization tandem triple quadrupole mass spectrometry in the multiple reaction monitoring mode. The experimental conditions for the extraction and analysis of phytohormones were optimized and validated in terms of reproducibility, linearity, sensitivity, recovery, accuracy, and stability. Distributions of the phytohormones in the stems, blades, and gas bladder of the S. horneri in drift, fixed, and semi‐fixed growing states were investigated for the first time. The observed contents of the phytohormones in S. horneri range from not detected to 5066.67 ng/g (fresh weight). Most phytohormones are distributed mainly in the stems of S. horneri in drift and semi‐fixed states.

更新日期：2016-05-03

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