新分离的根瘤菌对邻苯二甲酸酯的生物降解。LMB-1及其邻苯二甲酸二正丁酯的生化途径。,Journal of Applied Microbiology

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新分离的根瘤菌对邻苯二甲酸酯的生物降解。LMB-1及其邻苯二甲酸二正丁酯的生化途径。
Journal of Applied Microbiology ( IF 3.2 ) Pub Date : 2016 Mar 11 , DOI: 10.1111/jam.13123
W.-J. Tang ₁ , L.-S. Zhang ₁ , Y. Fang ₁ , Y. Zhou ₁ , B.-C. Ye ₁

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目的：分离一种可以有效降解多种PAE的新菌株，并研究该菌株的DBP降解途径。方法与结果：根据其16S rRNA基因序列，鉴定该菌株为根瘤菌。该菌株命名为LMB-1，也可以利用邻苯二甲酸酯，例如DEHP，DMP，DBP和DEP。在DBP降解过程中，通过气相色谱-质谱（GC-MS）分析确定了六种可能的代谢产物：邻苯二甲酸二乙酯，邻苯二甲酸单乙酯，邻苯二甲酸二甲酯，邻苯二甲酸单甲酯，邻苯二甲酸和酒石酸，本研究还确定了DBP的降解途径。结论：总的来说，发现菌株LMB-1，被鉴定为根瘤菌，能够有效降解PAEs，并且确定该菌株在45小时内完全降解了DMP。DEP，DMP，MEP，在LMB-1降解DBP的过程中检测到MMP，PA和酒石酸。我们建议这种菌株可以完全降解DBP或其他PAE。我们的结果提供了一种新颖且潜在的候选根瘤菌。LMB-1，用于生物修复受PAE污染的耕种土壤。研究的意义和影响：这是第一个关于根瘤菌完全降解邻苯二甲酸酯的报道。本文受版权保护。版权所有。这是关于根瘤菌完全降解邻苯二甲酸酯的首次报道。本文受版权保护。版权所有。这是关于根瘤菌完全降解邻苯二甲酸酯的首次报道。本文受版权保护。版权所有。

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Biodegradation of phthalate esters by newly isolated Rhizobium sp. LMB-1 and its biochemical pathway of di-n-butyl phthalate.

AIMS: To isolate a novel strain that could degrade many kinds PAEs efficiently and investigate the DBP degrading pathway in this strain. METHODS AND RESULTS: Based on its 16S rRNA gene sequence, the strain was identified as Rhizobium sp. This strain, named LMB-1, can also utilize phthalates, such as DEHP, DMP, DBP and DEP. During the degradation of DBP, six possible metabolites, diethyl phthalate, mono-ethyl phthalate, di-methyl phthalate, mono-methyl phthalate, phthalic acid, and tartaric acid, were identified by gas chromatography-mass spectrometry (GC-MS) analysis, and the degradation pathway of DBP was also identified in this study. CONCLUSIONS: In summary, strain LMB-1, identified as Rhizobium sp., was found to be capable of efficiently degrading PAEs, and it was determined that the strain degraded DMP completely within 45 h. DEP, DMP, MEP, MMP, PA and tartaric acid were detected during the course of DBP degradation by LMB-1. We propose that this strain could completely degrade DBP or other PAEs. Our results offer a novel and potential candidate, Rhizobium sp. LMB-1, for use in the bioremediation of cultivated soil contaminated by PAEs. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report concerning the complete degradation of phthalate esters by Rhizobium sp. This article is protected by copyright. All rights reserved.

更新日期：2017-01-31

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