POSS-纳米复合材料的表面改性，以增强合成生物支架的细胞整合。,Biomaterials

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POSS-纳米复合材料的表面改性，以增强合成生物支架的细胞整合。
Biomaterials ( IF 12.8 ) Pub Date : 2016 Mar , DOI: 10.1016/j.biomaterials.2016.01.005
Claire Crowley ₁ , Poramate Klanrit ₂ , Colin R Butler ₃ , Aikaterini Varanou ₃ , Manuela Platé ₄ , Robert E Hynds ₃ , Rachel C Chambers ₅ , Alexander M Seifalian ₂ , Martin A Birchall ₆ , Sam M Janes ₃

Affiliation

多面体低聚倍半硅氧烷聚（碳酸酯-脲）氨基甲酸酯 (POSS-PCU) 是一种多功能纳米复合生物材料，作为组织工程的生物支架应用越来越广泛。合成植入物与宿主组织的整合可能存在问题，但可以通过拓扑修改来改善。我们描述了通过将致孔剂（碳酸氢钠 (NaHCO3)、氯化钠 (NaCl) 和蔗糖）分散到材料表面来优化 POSS-PCU，其主要目的是增加表面孔隙率，从而为改善细胞和血管向内生长提供额外的机会. 我们评估了致孔剂对材料的机械强度、表面化学、润湿性和细胞相容性的影响。表面孔隙率通过扫描电子显微镜（SEM）表征。表面化学和润湿性没有变化，仅检测到机械性能的适度变化。致孔剂的大小与所生产的构建体的孔隙率很好地相关，并且较大的致孔剂改善了构建体内空间的互连性。使用原代人支气管上皮细胞 (HBEC)，我们展示了所有表面修饰的中等体外细胞相容性；然而，较大的孔导致细胞聚集。这些细胞能够在 POSS-PCU 支架上分化。体内支架的植入表明较大的孔径有利于细胞整合和血管向内生长。

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Surface modification of a POSS-nanocomposite material to enhance cellular integration of a synthetic bioscaffold.

Polyhedral oligomeric silsesquioxane poly(carbonate-urea) urethane (POSS-PCU) is a versatile nanocomposite biomaterial with growing applications as a bioscaffold for tissue engineering. Integration of synthetic implants with host tissue can be problematic but could be improved by topographical modifications. We describe optimization of POSS-PCU by dispersion of porogens (sodium bicarbonate (NaHCO3), sodium chloride (NaCl) and sucrose) onto the material surface, with the principle aim of increasing surface porosity, thus providing additional opportunities for improved cellular and vascular ingrowth. We assess the effect of the porogens on the material's mechanical strength, surface chemistry, wettability and cytocompatibilty. Surface porosity was characterized by scanning electron microscopy (SEM). There was no alteration in surface chemistry and wettability and only modest changes in mechanical properties were detected. The size of porogens correlated well with the porosity of the construct produced and larger porogens improved interconnectivity of spaces within constructs. Using primary human bronchial epithelial cells (HBECs) we demonstrate moderate in vitro cytocompatibility for all surface modifications; however, larger pores resulted in cellular aggregation. These cells were able to differentiate on POSS-PCU scaffolds. Implantation of the scaffold in vivo demonstrated that larger pore sizes favor cellular integration and vascular ingrowth. These experiments demonstrate that surface modification with large porogens can improve POSS-PCU nanocomposite scaffold integration and suggest the need to strike a balance between the non-porous surfaces required for epithelial coverage and the porous structure required for integration and vascularization of synthetic scaffolds in future construct design.

更新日期：2017-01-31

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