Layer-By-Layer Decorated Nanoparticles with Tunable Antibacterial and Antibiofilm Properties against Both Gram-Positive and Gram-Negative Bacteria,ACS Applied Materials & Interfaces

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Layer-By-Layer Decorated Nanoparticles with Tunable Antibacterial and Antibiofilm Properties against Both Gram-Positive and Gram-Negative Bacteria
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2018-01-09 00:00:00 , DOI: 10.1021/acsami.7b16508
Aleksandra Ivanova ₁ , Kristina Ivanova ₁ , Javier Hoyo ₁ , Thomas Heinze ₂ , Susana Sanchez-Gomez ₃ , Tzanko Tzanov ₁

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Bacteria-mediated diseases are a global healthcare concern due to the development and spread of antibiotic-resistant strains. Cationic compounds are considered membrane active biocidal agents having a great potential to control bacterial infections, while limiting the emergence of drug resistance. Herein, the versatile and simple layer-by-layer (LbL) technique is used to coat alternating multilayers of an antibacterial aminocellulose conjugate and the biocompatible hyaluronic acid on biocompatible polymer nanoparticles (NPs), taking advantage of the nanosize of these otherwise biologically inert templates. Stable polyelectrolyte-decorated particles with an average size of 50 nm and ζ potential of +40.6 mV were developed after five LbL assembly cycles. The antibacterial activity of these NPs against the Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli increased significantly when the polycationic aminocellulose was in the outermost layer. The large number of amino groups available on the particle surface, together with the nanosize of the multilayer conjugates, improved their interaction with bacterial membrane phospholipids, leading to membrane disruption, as confirmed by a Langmuir monolayer model, and the 10 logs reduction for both bacteria. The biopolymer decorated NPs were also able to inhibit the biofilm formation of S. aureus and E. coli by 94 and 40%, respectively, without affecting human cell viability. The use of LbL-coated NPs appears to be a promising antibiotic-free alternative for controlling bacterial infections using a low amount of antimicrobial agent.

中文翻译：

具有针对革兰氏阳性和革兰氏阴性细菌可调节的抗菌和抗生物膜特性的多层装饰纳米颗粒

由于抗生素抗性菌株的发展和传播，细菌介导的疾病成为全球医疗保健关注的问题。阳离子化合物被认为是具有膜活性的杀生物剂，具有控制细菌感染的巨大潜力，同时限制了耐药性的出现。在此，利用这些简单的生物惰性模板的纳米尺寸，使用通用且简单的逐层（LbL）技术在生物相容性聚合物纳米颗粒（NPs）上交替涂覆抗菌性氨基纤维素共轭物和生物相容性透明质酸的多层膜。经过五个LbL组装循环后，形成了平均粒径为50 nm，ζ电位为+40.6 mV的稳定的聚电解质修饰的颗粒。这些NP对革兰氏阳性菌的抗菌活性当聚阳离子氨基纤维素位于最外层时，金黄色葡萄球菌和革兰氏阴性大肠杆菌显着增加。朗格缪尔单层模型证实，颗粒表面上可用的大量氨基以及多层共轭物的纳米尺寸改善了它们与细菌膜磷脂的相互作用，导致膜破裂，两种细菌均减少了10 log 。生物聚合物修饰的纳米颗粒还能够抑制金黄色葡萄球菌和大肠杆菌的生物膜形成。分别降低了94％和40％，而不会影响人类细胞的生存能力。使用LbL包被的NP似乎是使用少量抗菌剂控制细菌感染的有前途的无抗生素替代品。

更新日期：2018-01-09

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