Citrate Improves Collagen Mineralization via Interface Wetting: A Physicochemical Understanding of Biomineralization Control,Advanced Materials

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Citrate Improves Collagen Mineralization via Interface Wetting: A Physicochemical Understanding of Biomineralization Control
Advanced Materials ( IF 27.4 ) Pub Date : 2018-01-08 , DOI: 10.1002/adma.201704876
Changyu Shao ₁ , Ruibo Zhao ₁ , Shuqin Jiang ₁ , Shasha Yao ₁ , Zhifang Wu ₂ , Biao Jin ₁ , Yuling Yang ₁ , Haihua Pan ₃ , Ruikang Tang _{1,

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Affiliation

Biological hard tissues such as bones always contain extremely high levels of citrate, which is believed to play an important role in bone formation as well as in osteoporosis treatments. However, its mechanism on biomineralization is not elucidated. Here, it is found that the adsorbed citrate molecules on collagen fibrils can significantly reduce the interfacial energy between the biological matrix and the amorphous calcium phosphate precursor to enhance their wetting effect at the early biomineralization stage, sequentially facilitating the intrafibrillar formation of hydroxyapatite to produce an inorganic–organic composite. It is demonstrated experimentally that only collagen fibrils containing ≈8.2 wt% of bound citrate (close to the level in biological bone) can reach the full mineralization as those in natural bones. The effect of citrate on the promotion of the collagen mineralization degree is also confirmed by in vitro dentin repair. This finding demonstrates the importance of interfacial controls in biomineralization and more generally, provides a physicochemical view about the regulation effect of small biomolecules on the biomineralization front.

中文翻译：

柠檬酸盐通过界面润湿改善胶原蛋白的矿化作用：对生物矿化控制的理化理解

生物硬组织（例如骨骼）总是含有极高水平的柠檬酸盐，据信在柠檬酸盐的骨形成和骨质疏松症治疗中起着重要的作用。但是，其生物矿化的机理尚未阐明。在这里，发现胶原纤维上吸附的柠檬酸盐分子可以显着降低生物基质与无定形磷酸钙前体之间的界面能，以增强它们在生物矿化早期的润湿效果，从而有利于羟磷灰石的纤维内形成，从而产生羟基磷灰石。无机－有机复合材料。实验证明，只有含有≈8.2wt％的结合柠檬酸盐（接近生物骨骼中的水平）的胶原原纤维才能达到与天然骨骼中一样的完全矿化作用。体外牙本质修复也证实了柠檬酸盐对胶原矿化程度的促进作用。该发现证明了界面控制在生物矿化中的重要性，并且更一般地，提供了关于小生物分子对生物矿化前沿的调节作用的物理化学观点。

更新日期：2018-01-08

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