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Cobalt-Porphyrin-Platinum-Functionalized Reduced Graphene Oxide Hybrid Nanostructures: A Novel Peroxidase Mimetic System For Improved Electrochemical Immunoassay.
Scientific Reports ( IF 3.8 ) Pub Date : 2015-Oct-13 , DOI: 10.1038/srep15113 Jian Shu 1 , Zhenli Qiu 1 , Qiaohua Wei 1 , Junyang Zhuang 1 , Dianping Tang 1
Scientific Reports ( IF 3.8 ) Pub Date : 2015-Oct-13 , DOI: 10.1038/srep15113 Jian Shu 1 , Zhenli Qiu 1 , Qiaohua Wei 1 , Junyang Zhuang 1 , Dianping Tang 1
Affiliation
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5,10,15,20-Tetraphenyl-21H,23H-porphine cobalt flat stacking on the reduced graphene oxide with platinum nanoparticles (PtNPs/CoTPP/rGO) were first synthesized and functionalized with monoclonal rabbit anti-aflatoxin B1 antibody (anti-AFB1) for highly efficient electrochemical immunoassay of aflatoxin B1 (AFB1) in this work. Transmission electron microscopy (TEM), atomic force microscope (AFM) and spectral techniques were employed to characterize the PtNPs/CoTPP/rGO hybrids. Using anti-AFB1-conjugated PtNPs/CoTPP/rGO as the signal-transduction tag, a novel non-enzymatic electrochemical immunosensing system was designed for detection of target AFB1 on the AFB1-bovine serum albumin-functionalized sensing interface. Experimental results revealed that the designed immunoassay could exhibit good electrochemical responses for target analyte and allowed the detection of AFB1 at a concentration as low as 5.0 pg mL(-1) (5.0 ppt). Intra- and inter-assay coefficients of variation were below 10%. Importantly, the methodology was further validated for analyzing naturally contaminated or spiked blank peanut samples with consistent results obtained by AFB1 ELISA kit, thus providing a promising approach for quantitative monitoring of organic pollutants.
中文翻译：
钴-卟啉-铂功能化还原氧化石墨烯混合纳米结构：一种用于改进电化学免疫测定的新型过氧化物酶模拟系统。
首先合成了 5,10,15,20-四苯基-21H,23H-卟啉钴，在带有铂纳米粒子的还原氧化石墨烯 (PtNPs/CoTPP/rGO) 上进行平堆积，并用单克隆兔抗黄曲霉毒素 B1 抗体（抗 AFB1）进行功能化）在这项工作中对黄曲霉毒素 B1 (AFB1) 进行高效电化学免疫分析。采用透射电子显微镜 (TEM)、原子力显微镜 (AFM) 和光谱技术来表征 PtNPs/CoTPP/rGO 杂化物。使用抗 AFB1 缀合的 PtNPs/CoTPP/rGO 作为信号转导标签，设计了一种新型非酶电化学免疫传感系统，用于在 AFB1-牛血清白蛋白功能化传感界面上检测目标 AFB1。实验结果表明，设计的免疫分析法对目标分析物表现出良好的电化学响应，并允许检测浓度低至5.0 pg mL(-1) (5.0 ppt)的AFB1。测定内和测定间变异系数低于 10%。重要的是，该方法得到了进一步验证，可用于分析自然污染或加标的空白花生样品，与 AFB1 ELISA 试剂盒获得的结果一致，从而为有机污染物的定量监测提供了一种有前景的方法。
更新日期：2015-10-15
中文翻译：
钴-卟啉-铂功能化还原氧化石墨烯混合纳米结构：一种用于改进电化学免疫测定的新型过氧化物酶模拟系统。
首先合成了 5,10,15,20-四苯基-21H,23H-卟啉钴，在带有铂纳米粒子的还原氧化石墨烯 (PtNPs/CoTPP/rGO) 上进行平堆积，并用单克隆兔抗黄曲霉毒素 B1 抗体（抗 AFB1）进行功能化）在这项工作中对黄曲霉毒素 B1 (AFB1) 进行高效电化学免疫分析。采用透射电子显微镜 (TEM)、原子力显微镜 (AFM) 和光谱技术来表征 PtNPs/CoTPP/rGO 杂化物。使用抗 AFB1 缀合的 PtNPs/CoTPP/rGO 作为信号转导标签，设计了一种新型非酶电化学免疫传感系统，用于在 AFB1-牛血清白蛋白功能化传感界面上检测目标 AFB1。实验结果表明，设计的免疫分析法对目标分析物表现出良好的电化学响应，并允许检测浓度低至5.0 pg mL(-1) (5.0 ppt)的AFB1。测定内和测定间变异系数低于 10%。重要的是，该方法得到了进一步验证，可用于分析自然污染或加标的空白花生样品，与 AFB1 ELISA 试剂盒获得的结果一致，从而为有机污染物的定量监测提供了一种有前景的方法。
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