Rapid synthesis of phosphoric diester and phosphorothioate oligonucleotides in 1 micromol to 1 millimol scale has been achieved using PNT nucleoside phosphoramidites. Furthermore, facile synthesis of mixed backbone oligonucleotides (MBOs) as antisense agents can be carried out using PNT nucleoside phosphoramidites in conjunction with PNT nucleoside H-phosphonates and PNT nucleoside phosphonamidites. The versatility of the PNT group is further demonstrated by its use in the preparation of bioreversible oligonucleotide conjugates.

中文翻译：

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N-戊-4-烯酰基（PNT）基团作为通用核碱基保护剂：在快速，简便地合成寡核苷酸，类似物和缀合物中的应用

使用PNT核苷亚磷酰胺可实现1微摩尔至1毫摩尔规模的磷酸二酯和硫代磷酸酯寡核苷酸的快速合成。此外，可以使用PNT核苷亚磷酰胺与PNT核苷H-膦酸酯和PNT核苷亚磷酰胺结合来容易地合成作为反义剂的混合主链寡核苷酸（MBO）。PNT基团在制备生物可逆性寡核苷酸缀合物中的用途进一步证明了其多功能性。