Multiphase Oscillatory Flow Strategy for in Situ Measurement and Screening of Partition Coefficients,Analytical Chemistry

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Multiphase Oscillatory Flow Strategy for in Situ Measurement and Screening of Partition Coefficients
Analytical Chemistry ( IF 6.7 ) Pub Date : 2015-10-13 00:00:00 , DOI: 10.1021/acs.analchem.5b03311
Milad Abolhasani ₁ , Connor W. Coley ₁ , Klavs F. Jensen ₁

Affiliation

Taking advantage of the difference between the surface energies of aqueous and organic solvents on a Teflon substrate, a fully automated small-scale strategy is developed on the basis of gas-driven oscillatory motion of a biphasic slug for high-throughput in situ measurement and screening of partition coefficients of organic substances between aqueous and organic phases. The developed oscillatory flow strategy enables single partition coefficient data point measurement within 8 min (including the sample preparation time) which is 360 times faster than the conventional “shake-flask” method, while using less than a 30 μL volume of the two phases and 9 nmol of the target organic substance. The developed multiphase strategy is validated using a conventional shake-flask technique. Finally, the developed strategy is extended to include automated screening of partition coefficients at physiological temperature.

中文翻译：

原位测量和分配系数筛选的多相振荡流策略

利用特氟龙基材上水性和有机溶剂的表面能之间的差异，基于双相段塞的气体驱动振荡运动，开发了一种全自动小规模策略，以实现原位高通量测量和筛选有机物在水相和有机相之间的分配系数。发达的振荡流策略可在8分钟内（包括样品制备时间）在单个分配系数数据点上进行测量，这比传统的“摇瓶”方法快360倍，而两相的体积小于30μL。 9 nmol的目标有机物。使用常规摇瓶技术验证了开发的多相策略。最后，已开发的策略已扩展到包括在生理温度下自动筛选分配系数的方法。

更新日期：2015-10-13

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