Abstract Protein nanoparticles can be used to encapsulate, protect, and deliver hydrophobic bioactive agents however, they are often unstable to environmental stresses. In this study, the impact of electrostatic deposition of a biopolymer coating (alginate/gelatin) on the physicochemical and functional properties of curcumin-loaded zein nanoparticles was determined. The pH stability of the zein nanoparticles was greatly improved by the biopolymer coatings, with a 7:3 w/w alginate-to-gelatin ratio being the most effective at inhibiting particle aggregation from pH 3 to 7. The optimized biopolymer-coated nanoparticles remained stable to aggregation during long-term storage, and were resistant to heat treatment at 80 °C for up to 40 min in 10% aqueous sucrose solutions (pH 4.5). The in vitro bioaccessibility of curcumin was appreciably higher (22.4%) when it was encapsulated within biopolymer-coated zein nanoparticles than when it was not encapsulated (

中文翻译：

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使用生物聚合物涂层定制玉米醇溶蛋白纳米颗粒功能：在模拟胃肠道条件下对姜黄素生物可及性和抗氧化能力的影响

摘要 蛋白质纳米粒子可用于封装、保护和递送疏水性生物活性剂，但它们通常对环境压力不稳定。在这项研究中，确定了生物聚合物涂层（藻酸盐/明胶）的静电沉积对加载姜黄素的玉米醇溶蛋白纳米粒子的物理化学和功能特性的影响。生物聚合物涂层大大提高了玉米醇溶蛋白纳米颗粒的 pH 稳定性，7:3 w/w 的海藻酸盐与明胶的比率在抑制 pH 3 至 7 的颗粒聚集方面最有效。优化的生物聚合物涂层纳米颗粒仍然存在在长期储存期间对聚集稳定，并且在 10% 蔗糖水溶液（pH 4.5）中在 80°C 下热处理长达 40 分钟。姜黄素的体外生物可及性明显更高（22.