Intracellular Self-Assembly and Disassembly of 19F Nanoparticles Confer Respective “Off” and “On” 19F NMR/MRI Signals for Legumain Activity Detection in Zebrafish,ACS Nano

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Intracellular Self-Assembly and Disassembly of 19F Nanoparticles Confer Respective “Off” and “On” 19F NMR/MRI Signals for Legumain Activity Detection in Zebrafish
ACS Nano ( IF 15.8 ) Pub Date : 2015-04-15 00:00:00 , DOI: 10.1021/acsnano.5b00287
Yue Yuan , Shuchao Ge , Hongbin Sun ₁ , Xuejiao Dong , Hongxin Zhao ₁ , Linna An , Jia Zhang , Junfeng Wang ₁ , Bing Hu , Gaolin Liang

Affiliation

¹⁹F MRI has higher selectivity but lower sensitivity than ¹H MRI for in vivo diagnosis. Therefore, to avoid using a high injection dose of the ¹⁹F probe while, in the meantime, maintaining the high sensitivity of ¹⁹F MRI has remained challenging. Local self-assembly and disassembly of ¹⁹F nanoparticles could be one of the “smart” strategies to achieve this goal. Herein, we report a dual-functional probe 1 for glutathione (GSH)-controlled self-assembly and subsequent legumain (Lgmn)-controlled disassembly of its nanoparticles (i.e., 1-NPs). Self-assembly and disassembly of 1-NPs confer ¹⁹F magnetic resonance (MR) signals “off” and “on”, respectively. Employing this strategy, we successfully applied 1 for consecutive detections of GSH and Lgmn in vitro and in cells, imaging Lgmn activity in HEK 293T tumors in zebrafish at a low dosage under 14.1 T.

中文翻译：

¹⁹ F纳米粒子的细胞内自组装和拆卸分别提供“关”和“开”的¹⁹ F NMR / MRI信号，用于斑马鱼的豆科菌素活性检测

¹⁹ F MRI在体内诊断方面比¹ H MRI具有更高的选择性，但灵敏度却更低。因此，要避免使用高剂量的¹⁹ F探针，同时要保持¹⁹ F MRI的高灵敏度仍然具有挑战性。¹⁹ F纳米粒子的局部自组装和拆卸可能是实现此目标的“智能”策略之一。在本文中，我们报道了双功能探针1为谷胱甘肽（GSH）控制的自组装和随后的legumain（Lgmn）控制的拆卸其纳米颗粒（的即，1-的NP）。自组装和拆卸的1纳米粒胙¹⁹ F磁共振（MR）信号分别为“关”和“开”。采用这种策略，我们成功地应用了1在体外和细胞中连续检测GSH和Lgmn ，以低剂量在14.1 T以下的斑马鱼HEK 293T肿瘤中成像了Lgmn活性。

更新日期：2015-04-15

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