A ratiometric fluorescent probe 1 was synthesized, which exhibited high sensitivity and excellent selectivity for hydrazine recognition. Probe 1 can quantitatively detect hydrazine in the concentration range from 30 to 180 μM with a LOD of 2.1 μM. Furthermore, it displayed excellent selectivity, anti-interference over many nucleophilic species, biological species, metal ions and anions, and a fast response time (4 min). Due to its ability to target mitochondria, the response of hydrazine to this probe in a living cell was successfully tracked in a ratiometric manner via fluorescence imaging.

中文翻译：

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线粒体靶向的肼比例荧光检测，响应时间快†

合成了比例荧光探针1，该探针显示出高灵敏度和极好的肼识别选择性。探针1可以定量检测30至180μM浓​​度范围内的肼，LOD为2.1μM。此外，它显示出优异的选择性，对许多亲核物种，生物物种，金属离子和阴离子的抗干扰性，以及快速的响应时间（4分钟）。由于其靶向线粒体的能力，通过荧光成像成功地按比例跟踪了肼对活细胞中该探针的反应。