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Gold Nanoparticle‐Based Paper Sensor for Simultaneous Detection of 11 Benzimidazoles by One Monoclonal Antibody
Small ( IF 13.0 ) Pub Date : 2017-12-20 , DOI: 10.1002/smll.201701782 Lingling Guo 1, 2 , Xiaoling Wu 1, 2 , Liqiang Liu 1, 2 , Hua Kuang 1, 2 , Chuanlai Xu 1, 2
Small ( IF 13.0 ) Pub Date : 2017-12-20 , DOI: 10.1002/smll.201701782 Lingling Guo 1, 2 , Xiaoling Wu 1, 2 , Liqiang Liu 1, 2 , Hua Kuang 1, 2 , Chuanlai Xu 1, 2
Affiliation
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A colloidal gold immunochromatographic assay based on a generic monoclonal antibody is developed for the simultaneous detection of benzimidazoles and metabolite residues in milk samples. The monoclonal antibody is prepared using 2‐(methoxycarbonylamino)‐3H‐benzimidazole‐5‐carboxylic acid as the hapten, and it can recognize 11 types of benzimidazoles simultaneously. The immunochromatographic strip is assembled and labeled using gold nanoparticles. This strip can detect 11 benzimidazoles including albendazole, albendazole s‐oxide, albendazole sulfone, fenbendazole, fenbendazole sulfone, flubendazole, mebendazole, parbendazole, oxfendazole, oxibendazole, and carbendazim within 15 min in milk samples. Results are obtained visually with the naked eye, and the cutoff values and the visual limit of detection values for these benzimidazoles are 25, 6.25, 12.5, 12.5, 50, 25, 50, 50, 50, 6.25, and 25 ng mL−1, and 6.25, 3.125, 3.125, 1.56, 12.5, 6.25, 12.5, 12.5, 6.25, 0.78, and 12.5 ng mL−1, respectively. Results are also obtained using a hand‐held strip scan reader, with calculated limit of detection values for these benzimidazoles of 0.83, 0.77, 1.83, 0.98, 7.67, 3.50, 3.96, 5.71, 0.92, 0.59, and 1.69 ng mL−1, respectively. In short, the developed paper sensor is a useful tool for rapid and simple screening of residues of benzimidazoles in milk samples.
中文翻译：
基于金纳米颗粒的纸传感器，可通过一种单克隆抗体同时检测11种苯并咪唑
开发了一种基于通用单克隆抗体的胶体金免疫色谱分析方法，用于同时检测牛奶样品中的苯并咪唑和代谢物残留。单克隆抗体是使用2-（甲氧羰基氨基）-3H-苯并咪唑-5-羧酸作为半抗原制备的，它可以同时识别11种苯并咪唑。使用金纳米颗粒组装并标记免疫色谱条。该检测条可在15分钟内检测牛奶样品中的11种苯并咪唑，包括阿苯达唑，阿苯达唑，阿苯达唑砜，芬苯达唑，芬苯达唑砜，氟苯达唑，甲苯苯达唑，多苯达唑，奥芬达唑，奥苯达唑和多菌灵。肉眼可见结果，这些苯并咪唑的截断值和检测值的视觉极限分别为25、6.25，-1和6.25、3.125、3.125、1.56、12.5、6.25、12.5、12.5、6.25、0.78和12.5 ng mL -1。还可以使用手持式条带扫描读取器获得结果，这些苯并咪唑的检测极限计算值为0.83、0.77、1.83、0.98、7.67、3.50、3.96、5.71、0.92、0.59和1.69 ng mL -1，分别。简而言之，开发的纸张传感器是快速，简单地筛查牛奶样品中苯并咪唑残留物的有用工具。
更新日期：2017-12-20
中文翻译：
基于金纳米颗粒的纸传感器，可通过一种单克隆抗体同时检测11种苯并咪唑
开发了一种基于通用单克隆抗体的胶体金免疫色谱分析方法，用于同时检测牛奶样品中的苯并咪唑和代谢物残留。单克隆抗体是使用2-（甲氧羰基氨基）-3H-苯并咪唑-5-羧酸作为半抗原制备的，它可以同时识别11种苯并咪唑。使用金纳米颗粒组装并标记免疫色谱条。该检测条可在15分钟内检测牛奶样品中的11种苯并咪唑，包括阿苯达唑，阿苯达唑，阿苯达唑砜，芬苯达唑，芬苯达唑砜，氟苯达唑，甲苯苯达唑，多苯达唑，奥芬达唑，奥苯达唑和多菌灵。肉眼可见结果，这些苯并咪唑的截断值和检测值的视觉极限分别为25、6.25，-1和6.25、3.125、3.125、1.56、12.5、6.25、12.5、12.5、6.25、0.78和12.5 ng mL -1。还可以使用手持式条带扫描读取器获得结果，这些苯并咪唑的检测极限计算值为0.83、0.77、1.83、0.98、7.67、3.50、3.96、5.71、0.92、0.59和1.69 ng mL -1，分别。简而言之，开发的纸张传感器是快速，简单地筛查牛奶样品中苯并咪唑残留物的有用工具。
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