An enzymatic system for the synthesis of N-methyl-l-phenylalanine from phenylpyruvic acid and methylamine with a novel enzyme, N-methyl-l-amino acid dehydrogenase from Pseudomonas putida ATCC12633, using NADP⁺ and glucose dehydrogenase from Bacillus subtilis as a co-factor-recycling system is described. Analysis of the product on a laboratory preparative scale process revealed N-methyl-l-phenylalanine in 98% yield and over 99% ee. N-Methyl-l-phenylalanine can be used as chiral building blocks for the synthesis of several products with pharmacological activity.

中文翻译：

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由恶臭假单胞菌（Pseudomonas putida）的新型酶N-甲基-1-氨基酸脱氢酶酶促合成N-甲基-1-苯基丙氨酸

一种酶促体系，利用枯草芽孢杆菌的NADP ⁺和葡萄糖脱氢酶作为辅酶，利用一种新型酶（恶臭假单胞菌ATCC12633的N-甲基-1-氨基酸脱氢酶）从苯基丙酮酸和甲胺合成N-甲基-1-苯丙氨酸。描述了因子回收系统。在实验室制备规模方法上对产物的分析表明，N-甲基-1-苯丙氨酸的产率为98％，ee为99％以上。N-甲基-1-苯基丙氨酸可用作合成具有药理活性的几种产物的手性结构单元。