Self-Assembly of Ferritin Nanoparticles into an Enzyme Nanocomposite with Tunable Size for Ultrasensitive Immunoassay,ACS Nano

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Self-Assembly of Ferritin Nanoparticles into an Enzyme Nanocomposite with Tunable Size for Ultrasensitive Immunoassay
ACS Nano ( IF 15.8 ) Pub Date : 2015-10-06 00:00:00 , DOI: 10.1021/acsnano.5b03607
Dong Men _{1,

2} , Ting-Ting Zhang ₂ , Li-Wei Hou ₃ , Juan Zhou ₁ , Zhi-Ping Zhang ₁ , Yuan-Yuan Shi ₄ , Jin-Li Zhang ₅ , Zong-Qiang Cui ₁ , Jiao-Yu Deng ₁ , Dian-Bing Wang ₆ , Xian-En Zhang ₆

Affiliation

The self-assembly of nanoparticles into larger superstructures is a powerful strategy to develop novel functional nanomaterials, as these superstructures display collective properties that are different to those displayed by individual nanoparticles or bulk samples. However, there are increasing bottlenecks in terms of size control and multifunctionalization of nanoparticle assemblies. In this study, we developed a self-assembly strategy for construction of multifunctional nanoparticle assemblies of tunable size, through rational regulation of the number of self-assembling interaction sites on each nanoparticle. As proof-of-principle, a size-controlled enzyme nanocomposite (ENC) was constructed by self-assembly of streptavidin-labeled horseradish peroxidase (SA-HRP) and autobiotinylated ferritin nanoparticles (bFNP). Our ENC integrates a large number of enzyme molecules, together with a streptavidin-coated surface, allowing for a drastic increase in enzymatic signal when the SA is bound to a biotinylated target molecule. As result, a 10 000-fold increase in sensitivity over conventional enzyme-linked immunosorbent assays (ELISA) methods was achieved in a cardiac troponin immunoassay. Our method presented here should provide a feasible approach for constructing elaborate multifunctional superstructures of tunable size useful for a broad range of biomedical applications.

中文翻译：

铁蛋白纳米颗粒自组装成可调节尺寸的酶纳米复合物，用于超灵敏免疫测定

纳米粒子自组装成较大的超结构是开发新型功能纳米材料的有力策略，因为这些超结构显示出与单个纳米粒子或大量样品所显示的不同的集体特性。然而，在尺寸控制和纳米颗粒组件的多功能化方面存在越来越多的瓶颈。在这项研究中，我们通过合理调节每个纳米粒子上自组装相互作用位点的数量，开发了一种可调节尺寸的多功能纳米粒子组装体的自组装策略。作为原理证明，通过链霉亲和素标记的辣根过氧化物酶（SA-HRP）和自体生物素化的铁蛋白纳米颗粒（bFNP）的自组装，构建了大小受控的酶纳米复合材料（ENC）。我们的ENC整合了许多酶分子以及抗生蛋白链菌素包被的表面，当SA结合到生物素化的靶分子上时，酶信号会急剧增加。结果，在心脏肌钙蛋白免疫测定中，灵敏度比传统的酶联免疫吸附测定（ELISA）方法提高了1万倍。本文介绍的方法应为构建可调节尺寸的精细多功能上部结构提供一种可行的方法，该结构可用于广泛的生物医学应用。在心脏肌钙蛋白免疫测定中，灵敏度比传统的酶联免疫吸附测定（ELISA）方法提高了10,000倍。本文介绍的方法应为构建可调节尺寸的精细多功能上部结构提供一种可行的方法，该结构可用于广泛的生物医学应用。在心脏肌钙蛋白免疫测定中，灵敏度比传统的酶联免疫吸附测定（ELISA）方法提高了10,000倍。本文介绍的方法应为构建可调节尺寸的精细多功能上部结构提供一种可行的方法，该结构可用于广泛的生物医学应用。

更新日期：2015-10-06

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