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Effect of alcohol dehydrogenase‐1B and ‐7 polymorphisms on blood ethanol and acetaldehyde concentrations in healthy subjects with a history of moderate alcohol consumption
Drug Testing and Analysis ( IF 2.6 ) Pub Date : 2017-09-28 , DOI: 10.1002/dta.2251 Roberta Pastorino 1 , Luigi Iuliano 2 , Alessia Vecchioni 1 , Dario Arzani 1 , Mirta Milic 3, 4 , Francesca Annunziata 5 , Chiara Zerbinati 2 , Ettore Capoluongo 5 , Stefano Bonassi 3 , James D McKay 6 , Stefania Boccia 7
Drug Testing and Analysis ( IF 2.6 ) Pub Date : 2017-09-28 , DOI: 10.1002/dta.2251 Roberta Pastorino 1 , Luigi Iuliano 2 , Alessia Vecchioni 1 , Dario Arzani 1 , Mirta Milic 3, 4 , Francesca Annunziata 5 , Chiara Zerbinati 2 , Ettore Capoluongo 5 , Stefano Bonassi 3 , James D McKay 6 , Stefania Boccia 7
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This study aims to evaluate the effect of ADH1B and ADH7 genotypes on blood acetaldehyde and ethanol levels after alcohol ingestion, and to measure the genotoxic effect of smoking and ethanol on the buccal cells, also controlling for ADH variants. We recruited healthy Italian subjects with at least a moderate history of alcohol consumption. All subjects were given an alcoholic drink of 0.4 g ethanol /kg of body weight. Blood venous samples were collected at baseline, and 30, 60, 90, and 120 minutes after ingestion. Buccal cells were collected before ethanol ingestion. Sixty subjects were enrolled in the study. Individuals with the ADH1B GG genotype had median ethanol levels of 5.0mM (IQR 3.4–7.2), and those with the ADH1B GT/TT genotype had 4.7mM (IQR 4.2–4.8). Corresponding acetaldehyde levels were 1.5μM (IQR 0.7–2.6) for ADH1B GG genotype and 1.6μM (IQR 1.5–1.7) for ADH1B CG/GG genotype. Individuals with the ADH7 CC genotype had median ethanol levels of 5.0mM (IQR 3.3–7.2), while 5.0mM (IQR 4.7–5.6) was in those with the ADH7 CG/GG genotype. Corresponding acetaldehyde levels were 1.5 μM (IQR 0.7–2.6) for ADH7 CC genotype and 1.5 μM (IQR 1.4–1.6) for ADH7 CG/GG genotypes. A non‐significant increase in the frequency of karyolitic and pyknotic cells was found in the group of heavy drinkers and current smokers, when compared to the moderate drinkers and the non‐smokers. Our study does not support the hypothesis that ADH1B and ADH7 genotypes affect blood ethanol and acetaldehyde concentration.
中文翻译：
乙醇脱氢酶‐1B和‐7多态性对有适度饮酒史的健康受试者血液乙醇和乙醛浓度的影响
本研究旨在评估ADH1B和ADH7基因型对饮酒后血液乙醛和乙醇水平的影响，并测量吸烟和乙醇对口腔细胞的基因毒性作用，同时控制ADH变异。我们招募了至少有中度饮酒史的健康意大利受试者。所有受试者均饮用0.4克乙醇/公斤体重的酒精饮料。在基线以及摄入后 30、60、90 和 120 分钟收集静脉血样本。在摄入乙醇之前收集口腔细胞。六十名受试者参加了这项研究。具有ADH1B GG 基因型的个体的中位乙醇水平为 5.0mM (IQR 3.4–7.2)，而具有ADH1B GT/TT 基因型的个体的中位乙醇水平为 4.7mM (IQR 4.2–4.8)。 ADH1B GG 基因型的相应乙醛水平为 1.5μM (IQR 0.7–2.6)， ADH1B CG/GG 基因型的相应乙醛水平为 1.6μM (IQR 1.5–1.7)。具有ADH7 CC 基因型的个体的中位乙醇水平为 5.0 mM (IQR 3.3–7.2)，而具有ADH7 CG/GG 基因型的个体的中位乙醇水平为 5.0 mM (IQR 4.7–5.6)。 ADH7 CC 基因型的相应乙醛水平为 1.5 μM (IQR 0.7–2.6)， ADH7 CG/GG 基因型的相应乙醛水平为 1.5 μM (IQR 1.4–1.6)。与中度饮酒者和不吸烟者相比，重度饮酒者和当前吸烟者的核核细胞和固缩细胞频率没有显着增加。我们的研究不支持ADH1B和ADH7基因型影响血液乙醇和乙醛浓度的假设。
更新日期：2017-09-28
中文翻译：
乙醇脱氢酶‐1B和‐7多态性对有适度饮酒史的健康受试者血液乙醇和乙醛浓度的影响
本研究旨在评估ADH1B和ADH7基因型对饮酒后血液乙醛和乙醇水平的影响，并测量吸烟和乙醇对口腔细胞的基因毒性作用，同时控制ADH变异。我们招募了至少有中度饮酒史的健康意大利受试者。所有受试者均饮用0.4克乙醇/公斤体重的酒精饮料。在基线以及摄入后 30、60、90 和 120 分钟收集静脉血样本。在摄入乙醇之前收集口腔细胞。六十名受试者参加了这项研究。具有ADH1B GG 基因型的个体的中位乙醇水平为 5.0mM (IQR 3.4–7.2)，而具有ADH1B GT/TT 基因型的个体的中位乙醇水平为 4.7mM (IQR 4.2–4.8)。 ADH1B GG 基因型的相应乙醛水平为 1.5μM (IQR 0.7–2.6)， ADH1B CG/GG 基因型的相应乙醛水平为 1.6μM (IQR 1.5–1.7)。具有ADH7 CC 基因型的个体的中位乙醇水平为 5.0 mM (IQR 3.3–7.2)，而具有ADH7 CG/GG 基因型的个体的中位乙醇水平为 5.0 mM (IQR 4.7–5.6)。 ADH7 CC 基因型的相应乙醛水平为 1.5 μM (IQR 0.7–2.6)， ADH7 CG/GG 基因型的相应乙醛水平为 1.5 μM (IQR 1.4–1.6)。与中度饮酒者和不吸烟者相比，重度饮酒者和当前吸烟者的核核细胞和固缩细胞频率没有显着增加。我们的研究不支持ADH1B和ADH7基因型影响血液乙醇和乙醛浓度的假设。
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