All of the four nucleobases in DNA have replaced the 4-hydroxy group of N-[2-(tert-butoxycarbonylaminomethyl)-trans-4-hydroxy] tetrahydropyrrole acetic acid methyl ester with cis -stereochemistry. An efficient route for the synthesis of N-[2-(tert-butoxycarbonylaminomethyl)-trans-4-hydroxy]-tetrahydropyrrole acetic acid methyl ester has been developed. Starting with this intermediate, the protected monomers were synthesized by the Mitsunobu reaction or via its tosylate.

中文翻译：

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新型肽核酸单体的设计与合成

DNA中的所有四个核碱基均已用顺式-立体化学取代了N- [2-（叔丁氧基羰基氨基甲基）-反式-4-羟基]四氢吡咯乙酸甲酯的4-羟基。已经开发了合成N- [2-（叔丁氧基羰基氨基甲基）-反式-4-羟基]-四氢吡咯乙酸甲酯的有效途径。从该中间体开始，通过Mitsunobu反应或通过其甲苯磺酸酯合成被保护的单体。