来自嗜冷海洋细菌Colwellia psychrerythraea34H 的独特荚膜多糖结构，可模拟抗冻（糖）蛋白,Journal of the American Chemical Society

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来自嗜冷海洋细菌Colwellia psychrerythraea34H 的独特荚膜多糖结构，可模拟抗冻（糖）蛋白
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2015-01-02 , DOI: 10.1021/ja5075954
Sara Carillo ₁ , Angela Casillo ₁ , Giuseppina Pieretti ₁ , Ermenegilda Parrilli ₁ , Filomena Sannino _{1,

2} , Maddalena Bayer-Giraldi ₃ , Sandro Cosconati ₄ , Ettore Novellino ₅ , Marcela Ewert ₆ , Jody W. Deming ₆ , Rosa Lanzetta ₁ , Gennaro Marino ₁ , Michelangelo Parrilli ₁ , Antonio Randazzo ₅ , Maria L. Tutino ₁ , M. Michela Corsaro ₁

Affiliation

极地地区和高海拔环境的低温，特别是冰冷的栖息地，给许多微生物带来了挑战。它们在低于冰点条件下生活的能力意味着产生具有耐低温性的化合物。Colwellia psychrerythraea 34H 是一种从零度以下北极海洋沉积物中分离的γ-变形菌，为研究寒冷环境中的生命提供了模型。我们在这里报告了来自 C. psychrerythraea 34H 细胞的荚膜多糖的鉴定和详细的分子一级和二级结构。当细胞被苯酚/水提取并通过一维和二维核磁共振谱以及化学分析表征时，聚合物在水层中分离。还进行了分子力学和动力学计算。该多糖由含有两个氨基糖和两个糖醛酸的四糖重复单元组成，并带有苏氨酸作为取代基。这种独特的多糖的结构特征类似于存在于抗冻蛋白和糖蛋白中的结构特征。这些结果表明胶囊结构与 C. psychrerythraea 在冰点以下的海洋环境中定殖的能力之间可能存在相关性。

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A Unique Capsular Polysaccharide Structure from the Psychrophilic Marine BacteriumColwellia psychrerythraea34H That Mimics Antifreeze (Glyco)proteins

The low temperatures of polar regions and high-altitude environments, especially icy habitats, present challenges for many microorganisms. Their ability to live under subfreezing conditions implies the production of compounds conferring cryotolerance. Colwellia psychrerythraea 34H, a γ-proteobacterium isolated from subzero Arctic marine sediments, provides a model for the study of life in cold environments. We report here the identification and detailed molecular primary and secondary structures of capsular polysaccharide from C. psychrerythraea 34H cells. The polymer was isolated in the water layer when cells were extracted by phenol/water and characterized by one- and two-dimensional NMR spectroscopy together with chemical analysis. Molecular mechanics and dynamics calculations were also performed. The polysaccharide consists of a tetrasaccharidic repeating unit containing two amino sugars and two uronic acids bearing threonine as substituent. The structural features of this unique polysaccharide resemble those present in antifreeze proteins and glycoproteins. These results suggest a possible correlation between the capsule structure and the ability of C. psychrerythraea to colonize subfreezing marine environments.

更新日期：2015-01-02

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