A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO),Journal of the American Chemical Society

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A Fast and Selective Near-Infrared Fluorescent Sensor for Multicolor Imaging of Biological Nitroxyl (HNO)
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2014-03-13 , DOI: 10.1021/ja500315x
Alexandra T Wrobel ₁ , Timothy C Johnstone , Alexandria Deliz Liang , Stephen J Lippard , Pablo Rivera-Fuentes

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The first near-infrared fluorescent turn-on sensor for the detection of nitroxyl (HNO), the one-electron reduced form of nitric oxide (NO), is reported. The new copper-based probe, CuDHX1, contains a dihydroxanthene (DHX) fluorophore and a cyclam derivative as a Cu(II) binding site. Upon reaction with HNO, CuDHX1 displays a five-fold fluorescence turn-on in cuvettes and is selective for HNO over thiols and reactive nitrogen and oxygen species. CuDHX1 can detect exogenously applied HNO in live mammalian cells and in conjunction with the zinc-specific, green-fluorescent sensor ZP1 can perform multicolor/multianalyte microscopic imaging. These studies reveal that HNO treatment elicits an increase in the concentration of intracellular mobile zinc.

中文翻译：

一种用于生物硝基 (HNO) 多色成像的快速选择性近红外荧光传感器

报道了第一个用于检测硝酰基 (HNO) 的近红外荧光开启传感器，硝酰 (HNO) 是一氧化氮 (NO) 的单电子还原形式。新的铜基探针 CuDHX1 包含一个二氢氧杂蒽 (DHX) 荧光团和一个作为 Cu(II) 结合位点的环芴衍生物。与 HNO 反应后，CuDHX1 在比色皿中显示出五倍的荧光开启，并且对 HNO 的选择性高于硫醇和活性氮和氧物种。CuDHX1 可以检测活哺乳动物细胞中外源应用的 HNO，并与锌特异性绿色荧光传感器 ZP1 结合使用，可以进行多色/多分析物显微成像。这些研究表明，HNO 处理引起细胞内流动锌浓度的增加。

更新日期：2014-03-13

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