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Substrate-Initiated Synthesis of Cell-Penetrating Poly(disulfide)s
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2013-02-04 , DOI: 10.1021/ja311961k Eun-Kyoung Bang 1 , Giulio Gasparini 1 , Guillaume Molinard 1 , Aurélien Roux 1 , Naomi Sakai 1 , Stefan Matile 1
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2013-02-04 , DOI: 10.1021/ja311961k Eun-Kyoung Bang 1 , Giulio Gasparini 1 , Guillaume Molinard 1 , Aurélien Roux 1 , Naomi Sakai 1 , Stefan Matile 1
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Lessons from surface-initiated polymerization are applied to grow cell-penetrating poly(disulfide)s directly on substrates of free choice. Reductive depolymerization after cellular uptake should then release the native substrates and minimize toxicity. In the presence of thiolated substrates, propagators containing a strained disulfide from asparagusic or, preferably, lipoic acid and a guanidinium cation polymerize into poly(disulfide)s in less than 5 min at room temperature at pH 7. Substrate-initiated polymerization of cationic poly(disulfide)s and their depolymerization with dithiothreitol causes the appearance and disappearance of transport activity in fluorogenic vesicles. The same process is further characterized by gel-permeation chromatography and fluorescence resonance energy transfer.
中文翻译：
细胞穿透性聚（二硫化物）的底物引发合成
表面引发聚合的经验教训可用于直接在自由选择的基材上生长细胞穿透性聚（二硫化物）。细胞摄取后的还原解聚应释放天然底物并将毒性降至最低。在硫醇化底物存在的情况下，含有来自芦笋的二硫化物或优选硫辛酸和胍鎓阳离子的繁殖剂在室温、pH 7 下在不到 5 分钟内聚合成聚（二硫化物）。 阳离子聚的底物引发聚合（二硫化物）及其与二硫苏糖醇的解聚导致荧光囊泡中转运活性的出现和消失。同一过程通过凝胶渗透色谱法和荧光共振能量转移进一步表征。
更新日期：2013-02-04
中文翻译：
细胞穿透性聚（二硫化物）的底物引发合成
表面引发聚合的经验教训可用于直接在自由选择的基材上生长细胞穿透性聚（二硫化物）。细胞摄取后的还原解聚应释放天然底物并将毒性降至最低。在硫醇化底物存在的情况下，含有来自芦笋的二硫化物或优选硫辛酸和胍鎓阳离子的繁殖剂在室温、pH 7 下在不到 5 分钟内聚合成聚（二硫化物）。 阳离子聚的底物引发聚合（二硫化物）及其与二硫苏糖醇的解聚导致荧光囊泡中转运活性的出现和消失。同一过程通过凝胶渗透色谱法和荧光共振能量转移进一步表征。
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