Bioactivity-guided fractionation on the leaves of Aleurites fordii led to the isolation of a new tigliane diterpene ester, 12-O-hexadecanoyl-7-oxo-5-ene-16-hydroxyphorbol-13-acetate (1) along with four known compounds, 12-O-hexadecanoyl-7-oxo-5-ene-phorbol-13-acetate (2), 12-O-hexadecanoyl-phorbol-13-acetate (3), 12-O-hexadecanoyl-16-hydroxyphorbol-13-acetate (4), and 12-O-hexadecanoyl-4-deoxy-4α-16-hydroxyphorbol-13-acetate (5). The structures of these compounds were determined by interpretation of NMR (1D and 2D) spectroscopic data and MS data. All the isolates were evaluated for their effects on the induction of IFN-γ in NK92 cells. Compounds 3 and 4 exhibited the most potent responses in IFN-γ induction, comparable to the positive control, phorbol 12-myristate 13-acetate (PMA).

生物活性的分馏法对Aleurites fordii的叶子进行了分离，从而分离出一种新的tigliane二萜酯，12 - O-十六烷酰基-7-氧代-5-烯-16-羟基佛波醇-13-乙酸酯（1）和四种已知化合物，12 - O-十六烷酰基-7-氧代-5-烯-phorbol-13-乙酸酯（2），12 - O-十六烷酰基-phorbol-13-乙酸酯（3），12 - O-十六烷酰基-16-羟基-phorphor-13乙酸甲酯（4），和12- ö -hexadecanoyl -4-脱氧4α-16-hydroxyphorbol -13-乙酸酯（5）。这些化合物的结构通过NMR（1D和2D）光谱数据和MS数据的解释来确定。评价所有分离物对诱导NK92细胞中IFN-γ的作用。化合物3和4在IFN-γ诱导中表现出最有效的反应，与阳性对照phorbol 12-肉豆蔻酸酯13-乙酸酯（PMA）相当。