Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution,Journal of the American Chemical Society

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Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2011-06-08 , DOI: 10.1021/ja2018477
Julie B. Rannes ₁ , Avgousta Ioannou ₁ , Simon C. Willies ₁ , Gideon Grogan ₂ , Carsten Behrens ₃ , Sabine L. Flitsch ₁ , Nicholas J. Turner ₁

Affiliation

A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans D-mannose (Man) and D-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.

中文翻译：

使用定向进化获得的半乳糖氧化酶的工程变体进行糖蛋白标记

定向进化方法已用于生成半乳糖氧化酶 (GOase) 的变体，该变体可以选择性地氧化糖蛋白上的聚糖。通过酶变体在聚糖 D-甘露糖 (Man) 和 DN-乙酰氨基葡萄糖 (GlcNAc) 上引入的醛功能然后可用于标记糖蛋白以及在其表面显示甘露糖苷的全细胞。

更新日期：2011-06-08

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