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Release Properties and Cellular Uptake in Caco-2 Cells of Size-Controlled Chitosan Nanoparticles
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2017-12-08 00:00:00 , DOI: 10.1021/acs.jafc.7b03627
Hyun Jeong Je 1 , Eun Suh Kim 1 , Ji-Soo Lee 1 , Hyeon Gyu Lee 1
Affiliation  

The influences of particle size on the physicochemical, release, and cellular uptake properties of chitosan nanoparticles (CSNPs) were investigated. Ionotropic CSNPs of different sizes (200–1000 nm) loaded with two model core materials (resveratrol or coumarin-6) were prepared using tripolyphosphate and carrageenan as cross-linkers. With an increase of particle size, zeta potential (34.6 ± 0.5 to 51.1 ± 0.9) and entrapment efficiency (14.9 ± 1.4 to 40.9 ± 1.9) of the CSNPs were significantly (p < 0.05) increased and release rates were decreased. However, Caco-2 cellular uptake of CSNPs were significantly increased from 3.70 ± 0.03 to 5.24 ± 0.20 with an increase of particle size from 200 to 600 nm, whereas those significantly decreased from 5.24 ± 0.20 to 4.55 ± 0.2 for particles larger than 600 nm in transwell assay. Moreover, much the same uptake patterns were also observed in confocal microscopy and flow cytometry. Investigation of cellular uptake of CSNPs revealed positive correlations between ZP and EE and indicated the effects of complex factors of nanoparticles other than size. These results provide a better understanding of CSNPs absorption and raises the possibility of controlling alternative nanoparticle properties to enhance bioavailability.

中文翻译:

大小控制的壳聚糖纳米颗粒的Caco-2细胞的释放特性和细胞摄取。

研究了粒径对壳聚糖纳米粒子(CSNPs)的理化,释放和细胞摄取特性的影响。使用三聚磷酸盐和角叉菜胶作为交联剂,制备了装载有两种模型核心材料(白藜芦醇或香豆素-6)的不同尺寸(200-1000 nm)的离子型CSNP。随着粒径的增加,CSNPs的zeta电位(34.6±0.5至51.1±0.9)和包封效率(14.9±1.4至40.9±1.9)显着(p<0.05)增加而释放速率降低。然而,随着粒径从200 nm增加到600 nm,Caco-2细胞对CSNPs的摄取从3.70±0.03显着增加到5.24±0.20,而对于大于600 nm的颗粒,其从5.24±0.20显着下降到4.55±0.2在transwell分析中。此外,在共聚焦显微镜和流式细胞仪中也观察到了几乎相同的摄取模式。对CSNPs细胞摄取的研究显示ZP与EE之间呈正相关,并表明了纳米粒子的复杂因素(而不是大小)的影响。这些结果提供了对CSNPs吸收的更好理解,并增加了控制替代纳米颗粒性质以增强生物利用度的可能性。
更新日期:2017-12-08
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