3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo,Advanced Science

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3D‐Bioprinted Osteoblast‐Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo
Advanced Science ( IF 14.3 ) Pub Date : 2017-11-24 , DOI: 10.1002/advs.201700550
Xinyun Zhai _{1,

2,

3} , Changshun Ruan ₁ , Yufei Ma ₁ , Delin Cheng ₁ , Mingming Wu ₁ , Wenguang Liu ₃ , Xiaoli Zhao ₁ , Haobo Pan ₁ , William Weijia Lu ₂

Affiliation

An osteoblast‐laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay ([Mg_5.34Li_0.66Si₈O₂₀(OH)₄]Na_{0.66, clay})/hyaluronic acid sodium salt (HA) bio‐inks, is developed by a two‐channel 3D bioprinting method. The novel biodegradable bio‐ink A, comprised of a poly(ethylene glycol) (PEG)–clay nanocomposite crosslinked hydrogel, is used to facilitate 3D‐bioprinting and enables the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) is applied as bio‐ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency. The cell‐laden PEG–clay constructs not only encapsulated osteoblasts with more than 95% viability in the short term but also exhibited excellent osteogenic ability in the long term, due to the release of bioactive ions (magnesium ions, Mg²⁺ and silicon ions, Si⁴⁺), which induces the suitable microenvironment to promote the differentiation of the loaded exogenous ROBs, both in vitro and in vivo. This 3D‐bioprinting method holds much promise for bone tissue regeneration in terms of cell engraftment, survival, and ultimately long‐term function.

中文翻译：

3D生物打印的成骨细胞纳米复合水凝胶结构具有诱导微环境，可在体外和体内促进细胞活力、分化和成骨

一种载有成骨细胞的纳米复合水凝胶结构，基于聚乙二醇二丙烯酸酯（PEGDA）/锂皂石 XLG 纳米粘土（[Mg _5.34 Li _0.66 Si ₈ O ₂₀ (OH) ₄ ]Na _{0.66，粘土}）/透明质酸钠盐（HA）生物墨水，是通过双通道3D生物打印方法开发的。新型可生物降解生物墨水 A 由聚乙二醇 (PEG)-粘土纳米复合交联水凝胶组成，用于促进 3D 生物打印，并能够有效地将氧气和营养物质输送到生长的细胞。 HA 与封装的原代大鼠成骨细胞 (ROB) 一起用作生物墨水 B，旨在提高细胞活力、分布均匀性和沉积效率。充满细胞的 PEG-粘土结构不仅在短期内封装成骨细胞的存活率超过 95%，而且由于释放生物活性离子（镁离子、Mg ²⁺和硅离子），从长远来看也表现出优异的成骨能力。 , Si ⁴⁺ )，诱导合适的微环境，促进加载的外源 ROB 在体外和体内的分化。这种 3D 生物打印方法在细胞植入、存活和最终长期功能方面为骨组织再生带来了很大希望。

更新日期：2017-11-24

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