To document the safety of pachybasin, a secondary metabolite of Trichoderma harzianum, for use as a bioagricultural agent, it was subjected to general toxicological testing in mice and developmental toxicity in zebrafish. With either 5 or 20 mg kg^–1 pachybasin i.p. injection, mice behavioral responses such as motor coordination, spontaneous locomotor activity, or nociceptive pain were not influenced. In long-term effect (daily injection for 14 days), the physiological, hematological, liver, and kidney functions were not altered either. Evidence for the developmental toxicity of pachybasin (10–100 μM) in 72-h exposure period was shown in zebrafish larvae, based on developmental retardation, impairment of chorion, and increase of mortality. In summary, there are no significant general toxicities presented in the pachybasin-treated adult male mice. However, the embryo-toxicity in aquatic biota should be taken into consideration during bioagricultural agent application.

中文翻译：

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厚皮草素对体内一般毒性和发育毒性的影响

为了证明作为生物农业制剂的哈茨木霉的次生代谢产物厚水碱的安全性，在小鼠中进行了一般毒理学测试，在斑马鱼中进行了发育毒性实验。5或20 mg kg ^–1Pachybasin腹腔注射后，小鼠的行为反应如运动协调，自发运动功能或伤害性疼痛均未受到影响。在长期效果（每天注射14天）中，生理，血液，肝和肾功能也没有改变。基于发育迟缓，绒毛膜损害和死亡率增加，斑马鱼幼虫在72 h暴露期中存在厚朴素（10-100μM）的发育毒性证据。总而言之，经厚皮素治疗的成年雄性小鼠没有明显的一般毒性。但是，在使用生物农业制剂时应考虑到水生生物的胚胎毒性。