Preparation, properties, formation mechanisms, and cytotoxicity of calcium oxalate monohydrate with various morphologies,CrystEngComm

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Preparation, properties, formation mechanisms, and cytotoxicity of calcium oxalate monohydrate with various morphologies
CrystEngComm ( IF 2.6 ) Pub Date : 2017-11-17 00:00:00 , DOI: 10.1039/c7ce01912b
Xin-Yuan Sun _{1,

2,

3,

4} , Chong-Yu Zhang _{1,

2,

3,

4} , Poonam Bhadja _{1,

2,

3,

4} , Jian-Ming Ouyang _{1,

2,

3,

4}

Affiliation

Calcium oxalate monohydrate (COM) crystals with various morphologies, such as elliptical, hexagonal, peanut-like, spherical and flower-like structures with a size of about 10 μm, were prepared through varying the reactant concentration, stirring speed, reaction temperature, and additive. X-ray diffraction and infrared spectroscopy showed that the synthesized crystals were pure-phase COM. The specific surface area and pore structure analysis demonstrated that all these COM crystals contained pores. Moreover, the zeta potential of each COM morphology was negative, and the value positively corresponded to the negative charge density of the crystal surface. Cell viability, lactate dehydrogenase (LDH) release, and reactive oxygen species (ROS) level assays suggested that these COM crystals caused injury to African green monkey renal epithelial (Vero) cells. Such an ability of the crystals to cause injury was correlated with the Ca²⁺ ion-rich ( [1 with combining macron]

01) crystal plane area, crystal edge sharpness, zeta potential and crystal-specific surface area. Hence, the cytotoxicity of the COM crystals was closely related to their morphology and surface structure.

中文翻译：

不同形态草酸钙一水合物的制备，性质，形成机理和细胞毒性

通过改变反应物的浓度，搅拌速度，反应温度和反应温度，制备出具有各种形态的草酸钙一水合物（COM）晶体，例如椭圆，六角形，花生状，球形和花状结构，大小约为10μm。添加剂。X射线衍射和红外光谱分析表明合成的晶体为纯相COM。比表面积和孔结构分析表明，所有这些COM晶体均包含孔。此外，每种COM形态的ζ电势为负，该值与晶体表面的负电荷密度正相关。细胞活力，乳酸脱氢酶（LDH）释放，活性氧（ROS）水平测定表明，这些COM晶体对非洲绿猴肾上皮（Vero）细胞造成了伤害。晶体造成伤害的这种能力与钙有关²⁺富离子（ [1个结合宏]