超高效液相色谱-三重四极杆质谱法检测马铃薯样品中甾体类生物碱,Analytical Methods

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超高效液相色谱-三重四极杆质谱法检测马铃薯样品中甾体类生物碱
Analytical Methods ( IF 2.7 ) Pub Date : 2017-10-18 00:00:00 , DOI: 10.1039/c7ay02244a
Xu-Heng Nie _{1,

2,

3,

4,

5} , Hua-Chun Guo _{1,

2,

3,

4}

Affiliation

优化和验证了一种超高效液相色谱-串联质谱（UHPLC-MS / MS）方法，以同时监测在自然室内条件下存储的马铃薯样品中的甾体类生物碱（SGA），α-查茄碱和α-茄碱在昆明。评估了两个不同的分析柱（Hypersil Gold C18和ZORBAX RRHD HILIC Plus）。ZORBAX RRHD HILIC Plus对SGA具有更好的分离效果。本方法中的样品直接用10％的乙酸水溶液萃取。马铃薯果肉和皮中SGA的回收率分别为86.3至92.3％和86.5至90.5％，精确度分别为2.2至4.5％和3.2至5.3％。日内和日间准确性验证数据在85.00至105.11％的范围内，变异系数（CV）为1.88至8。CV为2.11％至8.22％，分别为91％和98.50至100.59％。高相关系数（在²至400μgL ^-1 SGA下获得了α2-茄碱的R ² = 0.9997和α-茄碱的R ² = 0.9996 。结果表明，该方法的验证参数在美国食品药品监督管理局（USFDA）指南建议的可接受范围内。另外，皮肤中的SGA含量显着高于果肉中的SGA含量，并且随着时间的推移显着增加，但在马铃薯样品的果肉中却没有。

"点击查看英文标题和摘要"

An ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry method for the detection of steroidal glycoalkaloids in potato samples

An ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method has been optimized and validated to simultaneously monitor steroidal glycoalkaloids (SGAs), α-chaconine and α-solanine, in potato samples stored under natural indoor conditions in Kunming. Two different analytical columns (Hypersil Gold C18 and ZORBAX RRHD HILIC Plus) were assessed. The ZORBAX RRHD HILIC Plus showed better separation for the SGAs. The samples in the present method were directly extracted with 10% acetic acid aqueous solution. The recoveries of SGAs in the potato flesh and skin were in the range of 86.3 to 92.3% and 86.5 to 90.5% with a precision of 2.2 to 4.5% and 3.2 to 5.3%, respectively. The intra- and inter-day validation data for accuracy were in the range of 85.00 to 105.11% with a coefficient of variation (CV) of 1.88 to 8.91% and 98.50 to 100.59% with a CV of 2.11 to 8.22%, respectively. High correlation coefficients (R² = 0.9997 for α-chaconine and R² = 0.9996 for α-solanine) were obtained at 2 to 400 μg L⁻¹ SGAs. The results showed that the validation parameters of the method were in the acceptable range as recommended by the United States Food and Drug Administration (USFDA) guidelines. In addition, the SGA contents were significantly higher in the skin than those in the flesh, and significantly increased with time in the skin, but not in the flesh of potato samples.

更新日期：2017-11-06

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