Antileishmanial Drug Miltefosine-dsDNA Interaction in situ Evaluation with a DNA-Electrochemical Biosensor,Electroanalysis

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Antileishmanial Drug Miltefosine-dsDNA Interaction in situ Evaluation with a DNA-Electrochemical Biosensor
Electroanalysis ( IF 2.7 ) Pub Date : 2017-11-02 , DOI: 10.1002/elan.201700581
W. B. S. Machini ₁ , A. M. Oliveira-Brett ₁

Affiliation

Leishmaniasis is one of the most important parasitic neglected disease. The electrochemical evaluation of the antileishmanial drug miltefosine-dsDNA interaction was investigated in incubated solutions and using dsDNA-electrochemical biosensors, following the changes in the oxidation peaks of guanosine and adenosine residues, and the occurrence of the free guanine residues, electrochemical signal. The electrochemical behaviour of miltefosine was also investigated, at a glassy carbon electrode, using cyclic, differential pulse and square wave voltammetry and no electrochemical redox processes were observed. The interaction mechanism of miltefosine-dsDNA occurs in two ways: independent of the dsDNA sequence, and leading to the condensation/aggregation of DNA strands, producing a rigid miltefosine-dsDNA complex structure, and a preferential interaction between the guanine hydrogen atoms in the C−G base pair and miltefosine, causing the release of guanine residues detected on the electrode surface. Miltefosine did not induce oxidative damage to DNA in the experimental conditions used.

中文翻译：

抗利什曼原虫药物米替福新-dsDNA 相互作用与 DNA 电化学生物传感器的原位评价

利什曼病是最重要的寄生虫病之一。根据鸟苷和腺苷残基氧化峰的变化以及游离鸟嘌呤残基、电化学信号的出现，在温育溶液中和使用 dsDNA 电化学生物传感器研究了抗利什曼药米替福新-dsDNA 相互作用的电化学评价。还研究了米替福新的电化学行为，在玻碳电极上，使用循环、差分脉冲和方波伏安法，没有观察到电化学氧化还原过程。米替福新-dsDNA 的相互作用机制以两种方式发生：独立于 dsDNA 序列，并导致 DNA 链的凝聚/聚集，产生刚性的米替福新-dsDNA 复合结构，CG 碱基对中的鸟嘌呤氢原子与米替福新之间的优先相互作用，导致在电极表面检测到鸟嘌呤残基的释放。在使用的实验条件下，米替福新不会对 DNA 造成氧化损伤。

更新日期：2017-11-02

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