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Cucurbituril and Azide Cofunctionalized Graphene Oxide for Ultrasensitive Electro-Click Biosensing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-10-30 00:00:00 , DOI: 10.1021/acs.analchem.7b03068 Tianxiang Wei 1, 2 , Tingting Dong 1 , Hong Xing 1 , Ying Liu 1 , Zhihui Dai 1, 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-10-30 00:00:00 , DOI: 10.1021/acs.analchem.7b03068 Tianxiang Wei 1, 2 , Tingting Dong 1 , Hong Xing 1 , Ying Liu 1 , Zhihui Dai 1, 3
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To achieve high selectivity and sensitivity simultaneously in an electrochemical biosensing platform, cucurbituril and azide cofunctionalized graphene oxide, a new functional nanomaterial that acts as a go-between to connect the recognition element with amplified signal architecture, is developed in this work. The cucurbituril and azide cofunctionalized graphene oxide features a high specific surface area with abundant levels of the two types of functional groups. Specifically, it emerges as a powerful tool to link recognition elements with simplicity, high yield, rapidity, and highly selective reactivity through azide-alkynyl click chemistry. Moreover, it possesses many host molecules to interact with guest molecules (also signal molecules)-grafted branched ethylene imine polymer, through which the detection sensitivity can be greatly improved. Together with electro-click technology, a highly controllable, selective, and sensitive biosensing platform can be easily created. For VEGF165 protein detection, the electro-click assay has high selectivity and sensitivity; a dynamic detection range from 10 fg mL–1 to 1 ng mL–1 with a detection limit of 8 fg mL–1 was achieved. The electro-click biosensing strategy based on cucurbituril and azide cofunctionalized graphene oxide would have great promise for other target analytes with a broad range of applications.
中文翻译：
葫芦素和叠氮化物共官能化氧化石墨烯用于超灵敏电点击生物传感
为了在电化学生物传感平台上同时实现高选择性和灵敏度，在这项工作中，开发了葫芦科素和叠氮化物共官能化氧化石墨烯，一种新型的功能性纳米材料，可作为连接识别元件和放大信号结构的中介。葫芦素和叠氮化物共官能化的氧化石墨烯具有高的比表面积和两种类型的官能团含量高的特征。具体而言，它是通过叠氮化物-炔基点击化学将识别元素与简单，高产率，快速和高度选择性反应性联系在一起的强大工具。此外，它具有许多宿主分子与客体分子（也包括信号分子）接枝的支链亚乙基亚胺聚合物相互作用，从而可以大大提高检测灵敏度。结合电子点击技术，可以轻松创建高度可控，选择性和灵敏的生物传感平台。对于VEGF165种蛋白质检测，电点击分析具有很高的选择性和灵敏度；动态检测范围从10 fg mL –1到1 ng mL –1，检测限为8 fg mL –1。基于葫芦素和叠氮化物共官能化氧化石墨烯的电子点击生物传感策略将对具有广泛应用范围的其他目标分析物具有广阔的前景。
更新日期：2017-10-31
中文翻译：
葫芦素和叠氮化物共官能化氧化石墨烯用于超灵敏电点击生物传感
为了在电化学生物传感平台上同时实现高选择性和灵敏度，在这项工作中，开发了葫芦科素和叠氮化物共官能化氧化石墨烯，一种新型的功能性纳米材料，可作为连接识别元件和放大信号结构的中介。葫芦素和叠氮化物共官能化的氧化石墨烯具有高的比表面积和两种类型的官能团含量高的特征。具体而言，它是通过叠氮化物-炔基点击化学将识别元素与简单，高产率，快速和高度选择性反应性联系在一起的强大工具。此外，它具有许多宿主分子与客体分子（也包括信号分子）接枝的支链亚乙基亚胺聚合物相互作用，从而可以大大提高检测灵敏度。结合电子点击技术，可以轻松创建高度可控，选择性和灵敏的生物传感平台。对于VEGF165种蛋白质检测，电点击分析具有很高的选择性和灵敏度；动态检测范围从10 fg mL –1到1 ng mL –1，检测限为8 fg mL –1。基于葫芦素和叠氮化物共官能化氧化石墨烯的电子点击生物传感策略将对具有广泛应用范围的其他目标分析物具有广阔的前景。
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