Silver nanoparticles were synthesized using Olive oil (O-AgNPs) as reducing as well as capping agent and extensively characterized by UV–vis spectroscopy, fourier transform infrared, energy disperse spectroscopy, dynamic light scattering and atomic force microscopy. The chrome yellow color solution of O-AgNPs show the typical absorption maximum at 430 nm. FTIR analysis revealed that the carbonyl (CO) groups of Olive oil plays the most vital role in reduction of Ag⁺ and the nanoparticles synthesis. The morphology of O-AgNPs were found spherical in nature, while the size of O-AgNPs ranges between 35 to 65 nm as established by AFM and DLS studies, respectively. Stability of O-AgNPs studied by varying storage period, temperature, salt and pH of the medium were found quite stable probably because of oil suspension nature. Despite potent antimicrobial, anti-biofilm and biofilm eradicating activities, O-AgNPs were found to be non-toxic on cell lines. Moreover, chemosensing properties of the O-AgNPs were also tested against different drugs. The O-AgNPs showed high selectivity towards nitrofurazone (NFZ) with the lowest detection limit of 1.88 μM as was monitored by UV–vis spectroscopy. The mechanism of O-AgNPs interaction with drug was followed by DLS suggest that NFZ induces a time dependent nanoparticles aggregation.

银纳米颗粒是用橄榄油（O-AgNPs）作为还原剂和封端剂合成的，并通过紫外可见光谱，傅立叶变换红外光谱，能量分散光谱，动态光散射和原子力显微镜进行了广泛表征。O-AgNPs的铬黄色溶液在430 nm处显示出典型的最大吸收值。FTIR分析表明，橄榄油的羰基（CO）基团在还原Ag ⁺方面起着至关重要的作用。和纳米粒子的合成。通过AFM和DLS研究确定，O-AgNPs的形态在本质上是球形的，而O-AgNPs的大小在35至65 nm之间。通过改变介质的储存时间，温度，盐和pH研究的O-AgNPs的稳定性非常稳定，这可能是由于油的悬浮特性所致。尽管有有效的抗菌，抗生物膜和生物膜根除活性，但发现O-AgNP对细胞系无毒。此外，还针对不同药物测试了O-AgNP的化学传感特性。O-AgNPs对呋喃西林（NFZ）具有很高的选择性，最低检测限为1.88μM（通过紫外可见光谱法进行监测）。