<br>二甲基吡唑基硝化抑制剂作用于硝化和反硝化细菌，以减少 N2O 排放。,Scientific Reports

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二甲基吡唑基硝化抑制剂作用于硝化和反硝化细菌，以减少 N2O 排放。
Scientific Reports ( IF 3.8 ) Pub Date : 2017-Oct-23 , DOI: 10.1038/s41598-017-14225-y
Fernando Torralbo ₁ , Sergio Menéndez ₁ , Iskander Barrena ₁ , José M Estavillo ₁ , Daniel Marino _{1,

2} , Carmen González-Murua ₁

Affiliation

由于大量施氮 (N) 肥料，一氧化二氮 (N ₂ O) 排放量不断增加。土壤硝化和反硝化是N ₂ O 的主要来源，使用铵基肥料与硝化抑制剂（NI）相结合可有效减少农业系统的N ₂ O 排放。在这项工作中，我们研究了两种基于二甲基吡唑的 NI、3,4-二甲基吡唑磷酸盐 (DMPP) 和 2-(N-3,4-二甲基-1H-吡唑-1-基) 琥珀酸的_N2O减排能力异构混合物 (DMPSA)，对两种对比土壤含水量（40% 和 80% 土壤水填充孔隙空间；WFPS）下土壤硝化和反硝化微生物种群的影响。我们的结果表明，在 40% WFPS 条件下，DMPP 和 DMPSA 通过抑制细菌氨氧化来减少 N ₂ O 排放，效果相同。相比之下，在 80% WFPS 下，DMPSA 在减少 N ₂ O 排放方面的效率低于 DMPP。有趣的是，在 80% WFPS 下，氧气利用率降低限制了硝化作用，DMPP 和 DMPSA 不仅抑制硝化作用，而且还通过一氧化二氮还原酶活性（Nos 活性）刺激 N ₂ O 还原为分子氮 (N ₂ )。因此，在这项工作中，我们观察到基于 DMP 的 NI 在反硝化过程中刺激一氧化二氮还原酶将 N ₂ O 还原为 N ₂ 。

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Dimethyl pyrazol-based nitrification inhibitors effect on nitrifying and denitrifying bacteria to mitigate N2O emission.

Nitrous oxide (N₂O) emissions have been increasing as a result of intensive nitrogen (N) fertilisation. Soil nitrification and denitrification are the main sources of N₂O, and the use of ammonium-based fertilisers combined with nitrification inhibitors (NIs) could be useful in mitigating N₂O emissions from agricultural systems. In this work we looked at the N₂O mitigation capacity of two dimethylpyrazol-based NIs, 3,4-dimethylpyrazole phosphate (DMPP) and 2-(N-3,4-dimethyl-1H-pyrazol-1-yl) succinic acid isomeric mixture (DMPSA), on soil nitrifying and denitrifying microbial populations under two contrasting soil water contents (40% and 80% soil water filled pore space; WFPS). Our results show that DMPP and DMPSA are equally efficient at reducing N₂O emissions under 40% WFPS conditions by inhibiting bacterial ammonia oxidation. In contrast, at 80% WFPS DMPSA was less efficient than DMPP at reducing N₂O emissions. Interestingly, at 80% WFPS, where lowered oxygen availability limits nitrification, both DMPP and DMPSA not only inhibited nitrification but also stimulated N₂O reduction to molecular nitrogen (N₂) via nitrous oxide reductase activity (Nos activity). Therefore, in this work we observed that DMP-based NIs stimulated the reduction of N₂O to N₂ by nitrous oxide reductase during the denitrification process.

更新日期：2017-10-23

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