Whole bacterial cells containing stereospecific amidases were used for the kinetic resolution of racemic piperazine-2-carboxamide and piperidine-2-carboxamide to (S)- and (R)-piperazine-2-carboxylic acid, and (S)-piperidine-2-carboxylic acid, respectively. (S)-Piperazinecarboxylic acid dihydrochloride produced with the biocatalysts Klebsiella terrigena DSM 9174 had an ee value of 99.4% (41% yield), and the ee value of (R)-piperazinecarboxylic acid dihydrochloride obtained with Burkholderia sp. DSM 9925 was 99.0% (22% yield). Using Pseudomonas fluorescens DSM 9924 (S)-piperazine-2-carboxylic acid with an ee value of 97.3% (20% yield) was isolated.

中文翻译：

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通过在整个细菌细胞中用立体选择性酰胺酶动力学拆分相应的外消旋羧酰胺，制备（S）-哌嗪-2-羧酸，（R）-哌嗪-2-羧酸和（S）-哌啶-2-羧酸

含有立体特异性酰胺酶的整个细菌细胞用于消旋外消旋哌嗪-2-甲酰胺和哌啶-2-甲酰胺的动力学拆分为（S）-和（R）-哌嗪-2-羧酸和（S）-哌啶-2 -羧酸。用生物催化剂Klebsiella terrigena DSM 9174生产的（S）-哌嗪羧酸二盐酸盐的ee值为99.4％（收率41％），用Burkholderia sp。获得的（R）-哌嗪羧酸二盐酸盐的ee值。DSM 9925为99.0％（产率22％）。使用荧光假单胞菌DSM 9924（S分离出ee值为97.3％（产率为20％）的）-哌嗪-2-羧酸。