皮肤转录组揭示了自然和缩短光周期条件下绒山羊毛囊循环的内在分子机制。,Scientific Reports

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皮肤转录组揭示了自然和缩短光周期条件下绒山羊毛囊循环的内在分子机制。
Scientific Reports ( IF 3.8 ) Pub Date : 2017-Oct-18 , DOI: 10.1038/s41598-017-13986-w
Min Yang ₁ , Shen Song _{1,

2} , Kunzhe Dong ₁ , XiaoFei Chen ₁ , Xuexue Liu ₁ , Marhaba Rouzi ₁ , Qianjun Zhao ₁ , Xiaohong He ₁ , Yabin Pu ₁ , Weijun Guan ₁ , Yuehui Ma ₁ , Lin Jiang ₁

Affiliation

羊绒的生长呈现出由光周期变化引起的季节性模式。然而，潜在的分子机制仍不清楚。我们通过 RNA-seq 分析了六只山羊毛囊循环过程中七个时间点的皮肤转录组。这六只山羊包括三只暴露于自然光周期的山羊和三只暴露于缩短光周期的山羊。在毛发周期转变过程中，1713个基因表现出差异表达，332个基因表现出周期性表达模式。此外，短光周期诱导毛囊提早进入生长期，并且有246个基因与周期性基因重叠。在这些关键基因中，含有冷休克结构域的C2（CSDC2）在绒山羊皮肤的表皮和真皮中高表达，尽管其在毛囊发育中的功能仍不清楚。小鼠成纤维细胞中的 CSDC2 沉默导致两种关键毛囊因子的 mRNA 表达减少，从而导致细胞数量减少和细胞密度降低。羊绒的生长或蜕皮可能由一组周期性调节基因控制。适当的短时间光照管理可以通过激活这些调节因子来诱导毛囊尽早进入完全的毛发生长初期。CSDC2基因是毛发生长周期中潜在的重要转录因子。

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Skin transcriptome reveals the intrinsic molecular mechanisms underlying hair follicle cycling in Cashmere goats under natural and shortened photoperiod conditions.

The growth of cashmere exhibits a seasonal pattern arising from photoperiod change. However, the underlying molecular mechanism remains unclear. We profiled the skin transcriptome of six goats at seven time points during hair follicle cycling via RNA-seq. The six goats comprised three goats exposed to a natural photoperiod and three exposed to a shortened photoperiod. During hair cycle transition, 1713 genes showed differential expression, and 332 genes showed a pattern of periodic expression. Moreover, a short photoperiod induced the hair follicle to enter anagen early, and 246 genes overlapped with the periodic genes. Among these key genes, cold-shock domain containing C2 (CSDC2) was highly expressed in the epidermis and dermis of Cashmere goat skin, although its function in hair-follicle development remains unknown. CSDC2 silencing in mouse fibroblasts resulted in the decreased mRNA expression of two key hair-follicle factors, leading to reduced cell numbers and a lower cell density. Cashmere growth or molting might be controlled by a set of periodic regulatory genes. The appropriate management of short light exposure can induce hair follicles to enter full anagen early through the activation of these regulators. The CSDC2 gene is a potentially important transcription factor in the hair growth cycle.

更新日期：2017-10-18

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