The present study aimed to establish a simple and rapid method for the preparation of pure delphinidin-3-O-sambubioside (D3S) from Roselle and then investigate its antioxidant and hypoglycemic activity. According to our results, 60 mg of D3S with the purity of 96.1% was finally isolated from 500 g of Roselle by combination of macroporous resins and C₁₈ Sep-Pak cartridge techniques. Further studies revealed that D3S could significantly attenuate H₂O₂-induced oxidative stress by inhibiting H₂O₂-induced ROS overproduction, GSH depletion, mitochondrial membrane potential decrease and mitochondrial membrane peroxidation. In addition, we also found that D3S at the concentrations of 20 μg/mL and 40 μg/mL are able to promote glucose consumption and increase glycogen content in human liver HepG2 cells. These results indicated that D3S possesses potent antioxidant activity and hypoglycemic activity. Overall, our studies suggest that D3S could be a valuable material for designing functional foods against oxidative stress-related diseases and diabetes.

本研究旨在建立一种简单，快速的方法，以从Roselle中制备纯的delphinidin-3- O - sambubiobioside（D3S），然后研究其抗氧化和降血糖活性。根据我们的结果，通过大孔树脂和C ₁₈ Sep-Pak柱技术的结合，最终从500 g Roselle中分离出60 mg纯度为96.1％的D3S 。进一步的研究表明，D3S可以通过抑制H ₂ O ₂来显着减轻H ₂ O ₂引起的氧化应激。引起的ROS过度生产，GSH耗竭，线粒体膜电位降低和线粒体膜过氧化。此外，我们还发现浓度为20μg/ mL和40μg/ mL的D3S能够促进人肝HepG2细胞中的葡萄糖消耗并增加糖原含量。这些结果表明D3S具有有效的抗氧化活性和降血糖活性。总体而言，我们的研究表明D3S可能是设计针对与氧化应激相关疾病和糖尿病的功能性食品的有价值的材料。