便携式生物传感器，用于监测小排汗量中的皮质醇。,Scientific Reports

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便携式生物传感器，用于监测小排汗量中的皮质醇。
Scientific Reports ( IF 3.8 ) Pub Date : 2017-Oct-17 , DOI: 10.1038/s41598-017-13684-7
David Kinnamon , Ramesh Ghanta , Kai-Chun Lin , Sriram Muthukumar , Shalini Prasad

使用集成到纳米多孔柔性电极系统中的MoS ₂纳米片，证明了一种非法拉第无标签的皮质醇生物传感器。通过使用由垂直排列的金属电极限制半导体MoS ₂纳米片组成的新型传感器堆栈设计，实现了小体积（1-5μL）感测。使用皮质醇抗体对MoS ₂纳米片进行表面功能化，以开发出一种对出汗的人类皮质醇的生理相关范围（8.16至141.7 ng / mL）有特异性的亲和生物传感器。通过测量与沿MoS _2的皮质醇结合相关的阻抗变化来实现传感纳米片界面使用电化学阻抗谱。该传感器的动态范围为1-500 ng / mL，检出限为1 ng / mL。使用在人类汗液中表达的代谢物乙基葡萄糖醛酸苷进行了特异性研究。进行了连续剂量研究，在此过程中，传感器能够在3个多小时的时间内区分四个皮质醇浓度范围（0.5、5、50、500 ng / mL）。便携式形状因子设备显示了传感器的可翻译性，证明了该传感器具有可比的动态范围和检测极限。当将测量结果与台式仪器的报告阻抗值进行比较时，该设备的R ²相关值为0.998。

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Portable biosensor for monitoring cortisol in low-volume perspired human sweat.

A non-faradaic label-free cortisol biosensor was demonstrated using MoS₂ nanosheets integrated into a nanoporous flexible electrode system. Low volume (1-5 μL) sensing was achieved through use of a novel sensor stack design comprised of vertically aligned metal electrodes confining semi-conductive MoS₂ nanosheets. The MoS₂ nanosheets were surface functionalized with cortisol antibodies towards developing an affinity biosensor specific to the physiological relevant range of cortisol (8.16 to 141.7 ng/mL) in perspired human sweat. Sensing was achieved by measuring impedance changes associated with cortisol binding along the MoS₂ nanosheet interface using electrochemical impedance spectroscopy. The sensor demonstrated a dynamic range from 1-500 ng/mL with a limit of detection of 1 ng/mL. A specificity study was conducted using a metabolite expressed in human sweat, Ethyl Glucuronide. Continuous dosing studies were performed during which the sensor was able to discriminate between four cortisol concentration ranges (0.5, 5, 50, 500 ng/mL) for a 3+ hour duration. Translatability of the sensor was shown with a portable form factor device, demonstrating a comparable dynamic range and limit of detection for the sensor. The device demonstrated a R² correlation value of 0.998 when comparing measurements to the reported impedance values of the benchtop instrumentation.

更新日期：2017-10-17

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