The title compound was prepared enzymatically from l-lysine in an excellent yield and under buffer-free conditions. l-Lysine was oxidized by the action of l-lysine α-oxidase from Trichoderma viride followed by spontaneous oxidative decarboxylation of the intermediate 6-amino-2-oxocaproic acid in the reaction medium. l-Lysine α-oxidase was immobilized on an epoxy-activated solid support (Sepabeads EC-EP) and the activity of both solution-based and immobilized enzyme in this reaction was determined.

中文翻译：

---

有效的酶促合成5-氨基戊酸

由I-赖氨酸酶促制备标题化合物，产率高且在无缓冲液的条件下。通过来自木霉属木霉的1-赖氨酸α-氧化酶的作用将1-赖氨酸氧化，然后在反应介质中将中间体6-氨基-2-氧杂己酸自发地氧化脱羧。将1-赖氨酸α-氧化酶固定在环氧活化的固体支持物（Sepabeads EC-EP）上，并测定该反应中溶液基和固定酶的活性。