Enterobacter sp. CGMCC 2487, a bacterial strain isolated from the soil around a Taxus cuspidata Sieb. et Zucc. plant, was able to remove the xylosyl group from 7-xylosyltaxanes. The xylosidase of this strain was an inducible enzyme. In the bioconversion of 7-xylosyl-10-deacetyltaxol (7-XDT) to 10-deacetyltaxol (10-DT), for the purpose of enhancing the conversion efficiency, the effects of NH₄⁺, oat xylan, temperature, pH value, cell density and substrate concentration on the bioconversion have been systematically investigated. 3.0 mM NH₄⁺, 0.6% oat xylan in the media could enhance the yield of 10-DT; the optimum biocatalytic temperature was 26 °C and optimum pH value was 6.0. The highest conversion rate and yield of 10-DT from 7-XDT reached 92% and 764 mg/L, respectively. In addition, the biocatalytic capacity of the cell cultures remained 66.1% after continuous three batches. These results indicate that converting 7-XDT to 10-DT, a useful intermediate for the semisynthesis of paclitaxel or other taxane-based anticancer drugs by a novel bacterial strain, Enterobacter sp. CGMCC 2487, would be an alternative for the practical application in the future.

中文翻译：

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微生物将7-木糖基-10-去乙酰紫杉醇水解为10-去乙酰紫杉醇

肠杆菌属。CGMCC 2487，一种从红豆杉周围的土壤中分离出的细菌菌株。et Zucc。植物，能够从7-木糖基紫杉烷中除去木糖基。该菌株的木糖苷酶是可诱导的酶。在7-木糖基-10-去乙酰基紫杉醇（7-XDT）到10-去乙酰基紫杉醇（10-DT）的生物转化中，为了提高转化效率，NH ₄ ^+的作用，燕麦木聚糖，温度，pH值，已经系统地研究了细胞密度和底物浓度对生物转化的影响。3.0毫米NH ₄ ⁺在培养基中添加0.6％的燕麦木聚糖可以提高10-DT的产量；最佳生物催化温度为26°C，最佳pH值为6.0。7-XDT的10-DT最高转化率和产率分别达到92％和764 mg / L。另外，连续三批后，细胞培养物的生物催化能力保持66.1％。这些结果表明，将7-XDT转化为10-DT是一种新型细菌菌株Enterobacter sp。半合成紫杉醇或其他紫杉烷类抗癌药物的有用中间体。CGMCC 2487将是将来实际应用的替代方案。